外源基因在大肠杆菌中的诱导表达和降解物阻遏作用

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1、外源基因在大肠杆菌中的诱导表达和降解物阻遏作用Theinducedexpressionanddegradationofthegeneinescherichiacolibacteria2011-11-0309:42:55:bioxiucomment:0IwanttocommentExperimentnineexogenousgeneine.coliinducedexpressionandcataboliterepressionfunctionexperimentaim】【1.Understandtheexo

2、genousgeneinprokaryoticcellsexpressingthebasisofthetheory.Controlmechanismandoperationmethodoflactosemanipulation.1.Theexperimentprinciple】【proteinexpressionofforeigngenesinprokaryoticcellsusuallyistheultimategoaloftheresearch,thustheexpressionofproteinsi

3、ngeneticengineeringoccupiesveryimportant...Experimentalnineexogenousgeneswereusedtoinduceanddegradetheexpressionanddegradationofe.coli【experiment】Understandthebasictheoryexpressedbyforeigngenesintheprokaryoticcells.Controlmechanismandoperationmethodoflact

4、osemanipulation.【experimentalprinciple】TheexpressionofforeigngenesinprokaryoticcellsProteinisusuallytheultimategoalofresearch,sotheexpressionofproteinisveryimportantingeneticengineering.Commonexpressionsystemshaveprokaryoticcellsandeukaryoticcells.Theprok

5、aryoticcellexpressionsystemmainlyUSESe.coli,andtheeukaryoticexpressionsystemmainlyhasyeastcells,mammaliancellsandinsectcells.Theseexpressionsystemshavetheiradvantagesanddisadvantagesandshouldbeselectedaccordingtotheexperimentalandlaboratoryconditions.This

6、studymainlyintroducestheexpressionsystemofprokaryoticcellsrepresentedbye.coli.(1)e.coliexpressionsystemcharacteristics:Thebiologicalandgeneticbackgroundsareclearandeasytooperate;Thechoiceofmoreclonecarriershasbeendeveloped;Itiseasytoobtainlargeamountsofex

7、ogenousproteins(exogenousproteinsaccountforabout50%oftotalbacterialprotein).(2)theexpressionofproteinsintheprokaryoticcells:TheprokaryotehasitsownRNApolymerase,whichidentifiesthepromoteroftheprokaryote.Therefore,whenusingprokaryoticcellstoexpressthetarget

8、gene(whethertheeukaryoticgeneortheprokaryote),theoriginalnuclearpromotershouldbeused.ThemRNAoftheprokaryoticgenecontainsanSDsequencethatinitiatesthesynthesisofproteins.Ineukaryoticgenes,thereisnosuchsequence.Asaresu