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1、半枝莲联合反应停治疗肝癌的实验研究作者：李凌,徐雪明,李春来,程学新,万福生【摘要】目的通过BALB/C小鼠皮下接种肝癌H22细胞建立移植瘤模型,采用半枝莲乙醇提取液瘤内注射联合反应停治疗小鼠肝癌,探讨两药联合对肝癌移植瘤的抑制作用。方法体外培养小鼠肝癌H22细胞,取对数生长期细胞试验。乙醇回流提取半枝莲。接种H22细胞于BALB/C小鼠皮下建立肝癌H22的移植瘤模型。28只雄性BALB/C小鼠随机分为4组,每组7只。A组：反应停灌胃；B组：半枝莲乙醇提取液瘤内注射；C组：半枝莲乙醇提取液、反应停联合用药；D组：1%
2、乙醇0.2～0.5mL瘤内注射。观察肿瘤大小和瘤重,比较抑瘤效果。移植瘤采用抗CD31单抗做免疫组化染色,测定移植瘤组织微血管密度(MVD)。结果A、C组之间瘤重差异有统计学意义(P＜0.01),B、C组之间差异有统计学意义(P＜0.05)。A、B、C组抑瘤率分别为18.11%、53.15%和71.65%；协同系数0.74。3个治疗对照组之间微血管计数比较差异有统计学意义(P＜0.01),SNK-q检验显示A组和B组之间差异不明显(P＞0.05),A、B组分别与C组比较差异有统计学意义。结论半枝莲乙醇提取液瘤内注射联
3、合应用反应停对小鼠H22肝癌移植瘤有较强的抑制作用。半枝莲和反应停均有抗血管生成作用,两药联用对肿瘤微血管生成的抑制有协同作用。【关键词】半枝莲；反应停；肝肿瘤；抗血管生成；动物模型9Abstract：ObjectiveBALB/Cmicewereinoculatedsubcutaneouslywithhepatoma22(H22)cells,andthetreatmenteffectofthalidomideandintratumorinjectionofethanolextractofScutellariaBar
4、bata(SB)ontumorgrowthwereevaluated.MethodsInvitro,mouseH22livercellswerecultivated,andH22cellsinthelogarithmicgrowthphaseweretested.SBwasextractedintheethanolrecirculation.Atotalof28BALB/CmalemiceinoculatedsubcutaneouslywithH22cellswererandomlydividedinto4group
5、s,7micepergroup.GroupA：intragastricadministrationwiththalidomide.GroupB：SBintratumorinjection.GroupC：combinationofthalidomideandSBwiththesameadministrationasGroupAandGroupB.GroupD(controlgroup)：1%Ethanol0.2～0.5mLintratumorinjection.Tumorvolumesoffourgroupswereo
6、bservedandthetumorswereweighed.Themicrovesseldensities(MVD)ofalltransplantationtumorsweremeasuredbyimmunohistochemicalstainingwithanti-CD31monoclonalantibody.ResultsTherewerestatisticallysignificantdifferencesbetweenGroupCandGroupA(P＜0.01),orGroupB(P＜0.05).Thec
7、ooperativecoefficientwas0.74,meanwhiletheinhibititoryratesofGroupA,BandCwere18.11%,53.15%and71.65%,respectively.Measurementof9MicrovesselCount：TherewereStatisticallysignificantdifferencesamongthetreatedgroupsandthecontrolgroupD(P＜0.01),nostatisticallysignifican
8、tdifferencesbetweengroupAandB(P＞0.05).ThestudiesshowedstatisticallydifferencesbetweenGroupCandGroupA(P＜0.05),andbetweenGroupCandGroupB(P＜0.01),respectively.ConclusionsThecom
