吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤的保护作用

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1、吡咯烷二硫氨基甲酸酯对犬心肌线粒体损伤的保护作用作者：孟素峰，李有清，王启明【摘要】目的观察吡咯烷二硫氨基甲酸酯(PDTC)对体外循环(extracorporealcirculation,ECC)中犬心肌线粒体损伤的保护作用。方法12只犬随机分为对照组(C组,n=6)和PDTC组(P组,n=6)，腹腔内注射戊巴比妥钠25mg/kg麻醉后建立ECC心肌缺血再灌注模型。P组于ECC前静脉注射PDTC20mg/kg，C组静脉注射等量生理盐水。分别于ECC前、阻断主动脉60min、开放主动脉30min和60min时，测定心肌线粒体超氧化物歧化酶（SOD）、谷胱甘肽过氧化

2、物酶(GSH-PX）、丙二醛(MDA)、总抗氧化能力(T-AOC)和线粒体肿胀度(MSD)。结果与ECC前比较，两组阻断主动脉后SOD、GSH-PX及T-AOC均降低(P＜0.01)，MDA和MSD含量均升高；开放主动脉后C组SOD、GSH-PX及T-AOC均降低(P＜0.01)，MSD和MDA含量均升高(P＜0.01)；P组在开放主动脉60min时SOD、GSH-PX、T-AOC、MSD和MDA含量差异无统计学意义(P＞0.05)。P组在阻断主动脉60min时和开放主动脉后SOD、GSH-PX及T-AOC均显著高于C组(P＜0.01)，MSD和MDA含量均显著

3、低于C组(P＜0.01)。结论ECC中使用PDTC可提高心肌线粒体抗氧化能力，减轻心肌线粒体损伤。【关键词】10吡咯烷二硫氨基甲酸酯；线粒体；体外循环；心肌保护Abstract:OBJECTIVEToobservetheeffectofpyrrolidinedithiocarbamate(PDTC)oncaninemyocardialmitochondrialinjuryduringextracorporealcirculation(ECC).METHODSTwelvehealthymongreldogsundergoingECCwererandomlyallo

4、catedintocontrolgroup(groupC,n=6)andPDTCpretreatmentgroup(groupP,n=6).Alldogswereanesthesitizedwith2.5%sodiumpentobarbitone25mg/kg.Aftertrachealintubation,themyocardialischemia-reperfusioninjurymodelduringECCwasmadebydeclamping(DC)afteraorticcross-clamping(AC)for60min.IngroupP,PDTC20

5、mg/kgwasadministeredintravenouslybeforeECC，whileingroupCphysiologicalsalinewasgiveninsteadofPDTC.MyocardialmitochondriawereisolatedbeforeECC,60minafterAC，30minand60minafterDCfordeterminationofsuperoxidedismutase(SOD),glutathioneperoxidase(GSH-PX),malondialdehyde(MDA),totalanti-oxidat

6、ioncapacity(T-AOC)andmitochondrialswellingdegree(MSD).RESULTSThemyocardialmitochondriaofSOD,GSH-PXandT-AOCweredecreased，whileMDAandMSDwereincreasedafterACascomparedtothevaluebeforeECCinbothgroups(P＜0.01).SOD,GSH-PXandT-AOCweredecreased，whileMDAandMSDwereincreasedafterDC10ascomparedto

7、thevaluebeforeECCingroupC(P＜0.01).ThemyocardialmitochondriaofgroupPat60minafterDChadnotsignificantdifferencesincontentofMDA,SOD,GSH-PX,T-AOCandMSD(P＞0.05).SOD,GSH-PXandT-AOCweredistinctlylower，whileMDAandMSDweredistinctlyhigherat60minafterACandafterDCingroupPthanthoseingroupC(P＜0.01)

8、.CONCLUSIONP