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1、BMP异位诱导成骨的Micro-CT评价作者：李丹 刘民，胡蕴玉，毕龙，段永红，金静，王军，刘山金【摘要】借助Micro-CT评价单纯牛骨形态发生蛋白(bovinebonemorphogeneticprotein，bBMP)异位诱导成骨的长期三维影像学及骨质变化。(20±2)g昆明小鼠21只，麻醉后于双侧股部肌肉中植入bBMP各2mg，分别于1、2、4、6、8、10、12周各处死3只，切取诱导分化组织，5%戊二醛固定，行Micro-CT扫描和三维重建，运用ABA专用骨骼分析软件测定组织矿含量(tissuem
2、ineralcontent,TMC)，组织骨密度(tissuemineraldensity,TMD)，骨体积分数(bonevolumefraction,BVF)，结构模型指数(structuremodelindex,SMI),骨小梁厚度(trabecularthickness,Tb.Th),骨小梁数量(trabecularnumber,Tb.N)及皮质骨骨密度(bonemineraldensity,BMD)等参数，运用SPSS10.0统计软件进行统计学分析。bBMP从植入2周开始逐渐形成一椭圆形骨组织块，2
3、～4周，异位生成骨呈疏松的新生骨，4周时组织矿含量达第一个峰值，骨小梁数量最多;随着观察时间的延长(6～12周)，异位诱导生成的椭圆形骨组织内部骨小梁逐渐吸收，数量减少，12周时骨小梁数量最少;而外层骨组织逐渐塑形成为皮质骨，12周时骨矿含量值、骨小梁厚度、组织骨密度和皮质骨骨密度均达最大值。说明bBMP具有强大的异位骨诱导能力，血供不足时，骨质降解吸收;血供充足时，骨质逐渐成熟改建。12【关键词】骨形态发生蛋白;异位成骨;骨诱导;骨小梁;MicroCT Abstract：Toassessthe3-Dim
4、ageandchangesinqualityofosseoustissueproducedthroughectopicosteoinductionbybovinebonemorphegeneticproteinoveranextendedperiodbymeansofMicro-CT.Twenty-oneKunmingrats(20±2)gwereused，2mgbBMPwereimplantedintothighmusclesofbothsidesoftheratunderanesthesia.Three
5、animalsofeachweresacrificedat1,2,4,6,8,10and12weeksaftertheimplantation.Afterbeingharvested,thenewlyformedbonetissuewasfixedwith5%glutaraldehyde,thensuccessivelysubjectedtoMicro-CTscanand3-Dreconstruction,measurementoftissuemineralconsent(TMC)usingABAsoftw
6、areandtissuemineraldensity(TMD),determinationofbonevolumefraction(BVF),structuremodelindex(SMI),trabecularthickness(Tb.Th),trabecularnumber(Tb.N),corticalbonemineraldensity(BMD)andotherparameters,3-DimageswerecreatedandsavedasTIFFfiles;statisticalanalysesw
7、erecarriedoutbyANOVA(analysisofvariance)usingStatisticsPackageforSocialScience(SPSS)software.At2weekspostimplantation,anovalmassofosseoustissuebegantotakeshape;between2to4weeks,the12ectopic-inducednewbonepresentedasloosecancellousbonetissue,andthemineralco
8、ntentreachedthefirstpeakbythe4thweek,showingnumeroustrabeculae;theinnermosttrabecularoftheectopic-inducedlumpofosseoustissuewereresorbedwiththeirnumberdecreasedwithtime(between6thand12thweek),approachingthemi
