人脑星形细胞瘤组织中环氧化酶2的表达及其临床意义

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1、人脑星形细胞瘤组织中环氧化酶2的表达及其临床意义作者：徐滨，宋来君，刘献志，徐国本，张智峰【关键词】星形细胞瘤；前列腺素内过氧化物合酶；逆转绿聚合酶链反应；免疫组织化学　　Expressionandclinicalsignificanceofcyclooxygenase2inhumanastrocytoma　　【Abstract】AIM:Toinvestigatetheroleofcyclooxygenase(COX)inthedevelopmentandprogressionofhumanastrocytomasthroughexaminingtheexpressionsof

2、COX1andCOX2inhumanastrocytomatissueandnormalbraintissue.METHODS:Reversetranscriptasepolymerasechainreaction(RTPCR)andimmunohistochemistry(SP)wereappliedtodetectthemRNAandproteinexpressionsofCOX1andCOX2inhumanastrocytomatissueandnormalbraintissue.FurthemoretherelationshipbetweenCOXexpressionl

3、evelsandcancerpathologicalgradeswasanalyzed.RESULTS:UsingRTPCR,all20freshspecimensfromhumanastrocytomaswereshowntoexpressCOX2mRNA,whilenopositive12expressionofCOX2mRNAwasfoundin8specimensofnormalbraintissues;therewasasignificantdifferencebetweenthetwo(P<0.0001).ButCOX1mRNAwasexpressedcons

4、titutionallyinall20ofthefreshcancertissuesand8samplesofnormalbraintissues.TheimmunohistochemicalresultshowedthatCOX2proteinwasmainlylocalizedatcytoplasmofmalignantcells(positiveratewas72%),butwasnotexpressedinnormalbraintissues.Incontrast,COX1proteinwasmainlylocalizedatbraincellsinnormalbrai

5、ntissue(positiverate100%),butwasnotexpressedinhumanastrocytomas.In50paraffinembeddedspecimensofhumanastrocytomas,theintensityofCOX2expressionwasassociatedwithtumorgradeandstage.ThelevelofCOX2expressionwashigheringradeⅢ,ⅣtumorsthaningradeⅠ,Ⅱtumors.CONCLUSION:TheexpressionofCOX2mRNAandproteini

6、senhancedinhumanastrocytomasandisassociatedwithmalignantdegreeofthetumor,indicatingthatCOX2mayplayanimportantroleinthedevelopmentandprogressionofhumanastrocytomas.　　【Keywords】astrocytoma;prostaglandinendoperoxidesynthase;reversetranscriptasepolymerasechainreaction;immunohistochemistry12　　【摘要

7、】目的：通过检测环氧化酶1(COX1)和环氧化酶2(COX2)在人脑星形细胞瘤、正常脑组织中的表达，探讨COX在人脑星形细胞瘤发生发展中的作用.方法：应用逆转录聚合酶链反应(RTPCR)和免疫组化SP法检测人脑星形细胞瘤、正常脑组织中COX1,COX2mRNA和蛋白的表达及其与肿瘤恶性程度、病理级别的关系.结果：RTPCR检测20例人脑星形细胞瘤组织COX2mRNA均阳性表达，正常脑组织中未见表达，两者有显著性差异(P<0.0001)；COX1mRNA在20例肿瘤标本及正常脑组织中均有