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1、IL9在变应性鼻炎患者外周血单个核细胞中的表达水平作者:吉均祥钱炜邹晨马永明林志强陈建国杨先知王胜军【摘要】目的:检测变应性鼻炎患者IL9的表达水平,探讨IL9在变应性鼻炎发生发展中的意义。方法:收集49例变应性鼻炎患者的外周血标本,其中未治疗组30例,临床缓解组19例,另取30例健康志愿者的外周血标本作为正常对照组。采用实时荧光定量PCR(QRTPCR)检测IL9基因在外周血中的表达水平,酶联免疫吸附试验(ELISA)检测血浆IL9含量。结果:变应性鼻炎未治疗组外周血单个核细胞中IL9mRNA表达水平及血浆IL9含量明显高于变应性鼻炎
2、临床缓解组和正常对照组(P<0.05),而变应性鼻炎临床缓解组与正常对照组比较差异无统计学意义。变应性鼻炎未治疗组IL9mRNA表达水平与临床评分呈正相关。结论:变应性鼻炎患者外周血单个核细胞中IL9呈高表达。IL9可能参与了变应性鼻炎的病理过程,具体机制有待进一步探讨。【关键词】变应性鼻炎;白细胞介素9;实时荧光定量PCR ExpressionofIL9inperipheralbloodmononuclearcellwithallergicrhinitispatients13 JIJunxiang1,QIANWei1,ZOUCh
3、en1,MAYongming1,LINZhiqiang1,CHENJianguo1, YANGXianzhi2,WANGShengjun2 (1.DepartmentofOtolaryngology,theAffiliatedPeople'sHospitalofJiangsuUniversity,ZhenjiangJiangsu212002;2.DepartmentofImmunology,SchoolofMedicalScienceandLaboratoryMedicine,JiangsuUniversity,ZhenjiangJian
4、gsu212013,China) [Abstract]Objective:ToestablishamethodofrealtimefluorescencequantitativePCRfordetectingIL9mRNAinhumanperipheralbloodmononuclearcell(PBMC),andinvestigatetherelationshipbetweentheexpressionofIL9andallergicrhinitis(AR).Methods:ThemRNAlevelsofIL9inPBMCweredeter
5、minedbyquantitativerealtimePCR(QRTPCR),anddetectedtheconcentrationsofIL9inplasmabyenzymelinkedimmunosorbentassay(ELISA)in49patients,including30untreatedARpatientsand19patientsinrecoveringphase,and30healthycontrolvolunteers.Results:IL9mRNAexpressionandIL9concentrationsintheg
6、roupof30ARpatientswithout13treatmentweremarkedlyhigherthanthatinthegroupsof19ARpatientsinclinicalremissionand30healthycontrols(P<0.05).buttherewerenosignificantdifferencesbetweenthegroupsofARinclinicalremissionandnormalcontrol(P>0.05).Furthermore,theresultofthisstudyshowsa
7、positivelycorrelatedbetweenIL9mRNAandclinicalcountinuntreatedARpatients.Conclusion:TheseresultsindicatedthatIL9washigherinARpatientswithouttreatment,andsuggestingthatIL9mightbeinvolvedinthepathologicalprocessofAR.TheroleofIL9inprocessofARremainsfurtherinvestigation. [Keywor
8、ds]allergicrhinitis;IL9;realtimequantitativeP