apelin对两肾一夹高血压大鼠离体主动脉环的舒张作用

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1、Apelin对两肾一夹高血压大鼠离体主动脉环的舒张作用作者：庄红，金戈，陈磊，杨鹏麟【摘要】目的:探讨小分子活性肽Apelin-13对两肾一夹（2K1C）高血压大鼠离体主动脉环的舒张作用及意义。方法:①清洁级雄性SD大鼠27只，随机分为正常对照组和2K1C高血压模型组。测定平均颈动脉压(mCAP)、左心重与体重比值(LVM/BW)以及右肾重/左肾重(RK/LK)，以评价2K1C模型。②采用累计加药法，观察Apelin-13分别对用去甲肾上腺素（NE）预收缩的对照组大鼠和2K1C高血压组大鼠离体胸主动脉环张力的作用。结果:①模型组大鼠mCAP、LVM/BW和RK/LK分别较

2、对照组高50.38％(P<0.01)、32.16%(P<0.01)和51.02%(P<0.01)。②Apelin-13在（10-11～10-7）mol·L-1浓度范围内对内皮完整的两组大鼠胸主动脉环具有浓度依赖性的舒张作用，且模型组舒张率高于对照组(P<0.05)，而模型组与对照组EC50相比差异无显著性(P>0.05)。结论:Apelin-13具有显著的舒张主动脉的作用，并且对高血压大鼠血管环的舒张作用更强，提示Apelin-13在血管的保护作用中扮演了重要角色。【关键词】Apelin；主动脉；舒张作用；两肾一夹；高血压；肾性13Abstr

3、act:Objective:ToinvestigatetheeffectofapelinonvasodilatationofisolatedthoracicaortaringsinratsoftwoKindeyone-cliprenalhypertensionanditssignificance.Methods:①Twenty-sevenmaleSprague-Dawley（SD）ratswithweightof150～180gwererandomlydividedintotwogroups，controlrats(n=12)wasestimatedandmodelrat

4、softwoKindeyone-clip(2K1C)renalhypertension(n=15).Then，themodelof2K1Crenalhypertensionbymonitoringthemeancarotidarterialpressure(mCAP)，measuringtheratioofleftventricularmass(leftventricleplusseptum)tobodyweight(LVM/BW)，andrightkidneytotheleft(RK/LK).②Isolatedaortaringswereperfusedandwereg

5、iventherestingtension.Then，theeffectsofaccumulatedApelin-13onaortaringswithendotheliuminpre-constrictedwithnorepinephrine(NE)wereobserved.Results:①ThemCAPandLVM/BWof2K1Cgroupwere50.38%and32.16%higherthanthoseofthecontrolgroup，respectively(P<0.01，P<0.01).AndtheratioofRK/LKinthe2K1Cgr

6、oupwas51.02%higherthanthatinthecontrolgroup(P<0.01).②Apelin-13wasapotentvasodilatatoronratthoracicaorta.Apelin-13at10-11～10-7mol·L-1causeddose-depenentvasodilatationinendotheliumintactringspre-constrictedwithNE.Responseof13arteriesfromrenalhypertensionratswasincreased(P<0.05).Butthe

7、valuesofEC50werenotsignificantlydifferent(P>0.05).Conclusion:Theseresultsindicatethatapelin-13relaxesthethoracicaortainthetwogroupsandtheresponseofvasodilatationisenhancedintheratthoracicaortasfromthe2K1Chypertensiongroup.Itmaybeanimportantprotectingfactorandplay