核糖体印记技术[2篇]

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1、核糖体印记技术[2篇]以下是网友分享的关于核糖体印记技术的资料2篇，希望对您有所帮助，就爱阅读感谢您的支持。核糖体印记技术（一）InvestigateTranslationalControlviaRibosomeProfilingGeneCompany范丹青Translation?RemembertheCentralDogmaofMolecularBiology:hnRNA->mRNARegulation?EpigeneticsMolecularchaperonesRBPSilencingaminoacid

2、->polypeptide->proteinProteinlevels26areafunctionof:•RNAstability•Proteinstability•RegulationoftranslationTranslation?simplerulesaboutthenatureoftranslation•Ribosomecombinewith5’cap•ORFshaveaminimumlength•StartatthefirstAUG•BiasedcodonusageExceptions•IRES;

3、TEE•initiationatnon-AUGcodons•leakyscanning•translationalreinitiation•translationalframeshiftsWhatisRibosomeprofiling?ProteinsynthesisRibosometranslatingmRNAmRNARibosomeprofiling:isolateandsequenceonlythemRNAprotected/activelytranslatedbytheribosomeWhyisRi

4、bosomeProfilingImportant?Howcanyoutellhowmany26flowerswouldbethereviathenumberofbuds?No,youcannot.WhyisRibosomeProfilingImportant?HightranslationPoortranslationRibosomeProfilingvs.RNA-seqWorkflowdifferencesARTseq™RibosomeProfilingKitsLibraryPrepRNA-seqgive

5、sinformationONLYaboutmRNAabundanceRibosomeprofilingcanbeusedasaproxyforproteinexpression•DNAtoRNAtoProteinScriptSeq™Complete,TotalScript™RibosomeProfilingApplication►►►GenomewideinvestigationoftranslationinyeastDefinedactualproteinsequencesbeingtranslatedA

6、bsoluteproteinabundanceandresponsetoenvironmentalstressRibosomeProfilingApplicationRibosomeProfiling26Application►►►►DeterminingratesoftranslationelongationStrongpausesitesandunannotatedtranslationproductsuAUGandnon-AUGcodonsDefineaclassofshort,polycistron

7、icribosome-associatedcodingRNAs(sprcRNAs)thatencodesmallproteinsHarringtonine：三尖杉酯碱Cycloheximide：放线菌酮RibosomeProfilingApplicationsprcRNAs:short,polycistronicribosome-associatedcodingRNAsRibosomeProfilingApplication►MicroRNAsregulategeneexpression►Ribosomep

8、rofilingshowedthatmiR-430regulatestranslationinitiationbeforeinducingmRNAdecayduringzebrafishdevelopment.RibosomeProfilingApplication►MicroRNAsregulategeneexpression►RibosomeprofilingshowedthatmiR-430regulate