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1、酵母基因克隆与表达载体（Genecloningandexpressionvectorofyeast）Two.Yeastgenecloningandexpressionvector(a).Functionalskeletonvector-forfunctionalanalysisandvectorreconstructionfordifferentpurposes1,theyeastgenomeintegrationplasmid(Yeastintegratingplasmid,YIP)(1).T
2、rp(2).Urascreeningmarkerselectionmarker(3).Leu(4).Hisscreeningmarkerselectionmarker(5).G418screeningmarkerThisplasmidwaslinearizedtobeintegratedintotheyeastgenomePurpose:theintegrationofA.genedeletionofB.genesubstitutedorstableexpressionelement(integ
3、ration)andtype(knockin)2,yeastcentromeric-autonomouslyreplicatingplasmid(YeastCEN-ARSplasmid,YCP)(1).Trp(2).Urascreeningmarkerselectionmarker(3).Leu(4).Hisscreeningmarkerselectionmarker(5).G418screeningmarkerThisplasmidcouldselfreplicateincells,anddu
4、etothepresenceofcentriolereplicationsequence,soeachcellalmostonlyonecopy.Purpose:becauseeachcellaverageonlyonecopy,avoidtheexpressionoftargetgeneinsurplus,whichisespeciallysuitablefortheresearchofgenefunction3,yep(Yeastepisomal,plasmid,YEP)(1).Trp(2)
5、.Urascreeningmarkerselectionmarker(3).Leu(4).Hisscreeningmarkerselectionmarker(5).G418screeningmarkerThisplasmidreplicationincellswithhighlyepisomalform,eachcellisupto200-400acopyofbabePurpose:fortheexpressionofexogenousgene(two)expressionvector.1,GA
6、L1promoterexpressionvector,expressionvectorinducedbyD-galactose(1).Trp(2).Urascreeningmarkerselectionmarker(3).Leu(4).Hisscreeningmarkerselectionmarker(5).G418screeningmarker2,Cup1promoterexpressionvector,inducedbycoppersulfate(1).Trp(2).Urascreening
7、markerselectionmarker(3).Leu(4).Hisscreeningmarkerselectionmarker(5).G418screeningmarker3,ADHpromoterexpressionvector-ADC1constitutiveexpressionvector(1).Trp(2).Urascreeningmarkerselectionmarker(3).Leu(4).Hisscreeningmarkerselectionmarker(5).G418scre
8、eningmarkerYeastgeneticsresearchandgenefunction,higherbiologicalmagic(1)expressionofexogenousgeneinyeast2010-07-2411:51:11
9、classification:
10、labelmethodandyeastgenetics:yeastculturemediumselectiondefectofdropoutexpressionplasmid
11、report
12、namesubscription
