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ID:12724092
大小:3.81 MB
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时间:2018-07-18
《geo gene expression omnibus:geo基因表达数据库》由会员上传分享,免费在线阅读,更多相关内容在教育资源-天天文库。
1、GeneExpressionOmnibus(GEO)http://www.ncbi.nlm.nih.gov/geo/MicroarraysingeneexpressionAtypicalgeneexpressionanalysisexperiment:Differentialsystems–e.g.cancervs.normal,drugvs.nodrugChoose/preparethemicroarrayExtracttotalmRNA(transcriptome)Reversetranscription/dyelabelingCombineequalamountsHyb
2、ridizeScanANALYSEANDINTERPRETTHERESULTS–thisisthetrickypart…MicroarraysingeneexpressionSPOTSHybridizationSCANApublicrepositoryforthearchivinganddistributionofgeneexpressiondatasubmittedbythescientificcommunity.Curated,onlineresourceforgeneexpressiondatabrowsing,query,analysisandretrieval.Co
3、nvenientfordepositionofgeneexpressiondata,asrequiredbyfundingagenciesandjournals.GeneExpressionOmnibus(GEO):GeneExpressionandMolecularAbundanceDataRepositoryGEOArchitecturePlatform(GPL)=thetechnologyusedandthefeaturesdetected.Sample(GSM)=preparationanddescriptionofthesample.Series(GSE)defi
4、nesasetofsamplesandhowtheyarerelated.DataSets(GDS)sampledatacollectionsassembledbyGEOstaff.GEOhasfourkindsofdatarecordsGPLPlatformdescriptionsGSMRaw/processedspotintensitiesfromasingleslide/chipGSEGroupingofslide/chipdata“asingleexperiment”GDSGroupingofexperimentsCuratedbyNCBISubmittedbyExp
5、erimentalistsSubmittedbyManufacturerGEOArchitectureGEOHomePageSimpleinterfaceto:showstatusfinddocumentationquerydatabrowsedataPlatformAccessionPagePlatformdetails:DescriptionTypeOrganismManufacturerRelatedsamplesandseriesNOTETHETOPBAR(SCOPE)SampleAccessionPageSampledetails:DescriptionTypeOr
6、ganismSourceRelatedplatformsandseriesSampleFileDataTableDatatablefielddescriptionsTruncateddatatablefromQuickviewTotaldatarowsandfilesizeSeriesAccessionPageBiologicalsamplesummaryDesignsummaryPublicationinformationRelatedplatformsandsamplesDATASETorGENEPROFILEsearchGEOdatacanbequeriedas:Dat
7、asets:experiment-centricviewusingEntrezGEODataSetsGeneprofiles:gene-centricviewusingEntrezGEOProfilesSelectingeithertakesyoutoasimilarEntrezintroductionpageQueryingGEODataSetsStartaGEODataSetssearchwiththeQuery>DataSetstextboxThisbringsupanEntrezGEODataS
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