平访国立屏东教育大学97学年研究所硕士班入学考试

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1、國立屏東教育大學97學年度研究所碩士班入學考試生物化學試題（應用化學暨生命科學系碩士班）※請注意：1.本試題共四頁。2.答案須寫在答案卷，否則不予計分。Ⅰ.Multiplechoice(pleasechoosethebestanswer,2pointseach,30%)1.Onemethodusedtopreventdisulfidebondinterferencewithproteinsequencingproceduresis:A.cleavingproteinswithproteasesthatspecificallyrecognizedi

2、sulfidebonds.B.protectingthedisulfidebridgeagainstspontaneousreductiontocysteinylsulfhydrylgroups.C.reducingdisulfidebridgesandpreventingtheirre-formationbyfurthermodifyingthe—SHgroups.D.removingcystinesfromproteinsequencesbyproteolyticcleavage.E.sequencingproteinsthatdonotco

3、ntaincysteinylresidues.2.Inamixtureofthefiveproteinslistedbelow,whichshouldelutesecondinsize-exclusion(gel-filtration)chromatography?A.cytochromecMr=13,000B.immunoglobulinGMr=145,000C.ribonucleaseAMr=13,700D.RNApolymeraseMr=450,000E.serumalbuminMr=68,5003.Anahelixwouldbedesta

4、bilizedmostby:A.anelectricdipolespanningseveralpeptidebondsthroughouttheahelix.B.interactionsbetweenneighboringAspandArgresidues.C.interactionsbetweentwoadjacenthydrophobicValresidues.D.thepresenceofanArgresiduenearthecarboxylterminusoftheahelix.E.thepresenceoftwoLysresiduesn

5、eartheaminoterminusoftheahelix.4.Whichofthefollowingisnotcorrectconcerningcooperativebindingofaligandtoaprotein?A.Itisusuallyaformofallostericinteraction.B.Itisusuallyassociatedwithproteinswithmultiplesubunits.C.Itrarelyoccursinenzymes.D.ItresultsinanonlinearHillPlot.E.Itresu

6、ltsinasigmoidalbindingcurve.第4頁，共4頁Comparethetworeactioncoordinatediagramsbelowandselecttheanswerthatcorrectlydescribestheirrelationship.Ineachcase,thesingleintermediateistheEScomplex.A.(a)describesastrict“lockandkey”model,whereas(b)describesatransition-statecomplementaritymo

7、del.B.Theactivationenergyforthecatalyzedreactionis#5in(a)andis#7in(b).C.Theactivationenergyfortheuncatalyzedreactionisgivenby#5+#6in(a)andby#7+#4in(b).D.Thecontributionofbindingenergyisgivenby#5in(a)andby#7in(b).E.TheEScomplexisgivenby#2in(a)and#3in(b).2.Anenzyme-catalyzedrea

8、ctionwascarriedoutwiththesubstrateconcentrationinitiallyathousandtim