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1、1对脓毒症大鼠血浆应激激素和胰岛素样生长因子-1受体表达的影响中国临床营养杂志CHINESEJOURNALOFCLINICALNUTRITION胰岛素样生长因子-1对脓毒症大鼠血浆应激激素和胰岛素样生长因子.1受体表达的影响△高志光,刘敏丰,秦环龙#,沈通一(上海交通大学附属第六人民医院普通外科,上海200233)?论着?摘要:目的探讨胰岛素样生长因子.1(IGF.1)对脓毒症大鼠血浆应激激素及骨骼肌IGF.1受体(IGF一1R)蛋白质和mRNA表达的影响.方法选择71只SD大鼠采用盲肠结扎穿孔法建立
2、腹腔感染及颈静脉置管肠外营养(PN)模型后,将存活的30只大鼠随机分为脓毒症组(rg=10),PN组(rg=10)及IGF.1组(rg=10),另设正常组(rg=10).正常组和脓毒症组给予生理盐水,PN组给予营养支持,IGF.1组为PN+IGF.1静脉注射,连续5天.第6天行颈动脉置管,开腹取门静脉血测定胰岛素,胰高血糖素,肾上腺素,皮质醇和IGF.1水平;免疫组织化学法及实时定量PCR法测定肝脏和骨骼肌IGF.1R蛋白质和mRNA的表达.结果与对照组相比,脓毒症组大鼠血浆胰岛素,胰高血糖素,肾上腺
3、素,皮质醇水平显着升高,IGF一1显着下降(P<0.01);IGF.1组前5项指标较脓毒症组和PN组显着降低,IGF.1则显着升高(P<0.05,P<0.01).脓毒症组肝脏和骨骼肌IGF?1R蛋白质及mRNA表达较对照组显着增强(P<0.05,P<0.01);IGF一1组的IGF?1R蛋白质及mRNA表达较脓毒症组和PN组显着降低(P<0.01).结论IGF.1可改善应激激素之间的平衡,降低脓毒血症大鼠IGF.1R在蛋白质和mRNA水平的表达,从而有利于血糖的控制
4、.关键词:脓毒症;胰岛素抵抗;胰岛素样生长因子.I;应激激素;胰岛素样生长因子.1受体中图分类号:R459.3文献标识码:A文章编号:1008-5882(2006)05.0303.05EffectofInsulin-likeGrowthFactor-1ontheStressHormoneandInsulin.1ikeGrowthFactor-1ReceptorGeneExpressionandProteinContentinPyaemiaRats△GAOZhi-guang,LIUMin-feng,QI
5、NHuan-long,SHENTong.yi(DepartmentofSurgery,AffiliatedNo.6People'8HospitalofShahghaiJiaotongUniversity,Shanghai200233,China)ObjectiveToinvestigatetheeffectofinsulin-likegrowthfactor-1(IGF-1)onthestresshormone,IGF.1receptor(IGF-1R)proteinandmRNAexpression
6、.MethodsTotally71SDratswithabdominalinfectionmodelsestablishedbycecalligationandperforationmethodwererandomlydividedintothreegroups:pyaemicgroup(n=10),parenteralnutrition(PN)group(n=10),andIGF-1group(n=10).Another10SDratsweresetasthenormalcontrolgroup(n
7、:10).Onthesixthday,onesamplingcatheterinasinglecarotidwasperformed.Thevenacavabloodwassampledtodeterminethelevelsofinsulin,glucagon,adrenalin,cortico.steroid,andIGF-1.Inaddition,theexpressionoftheIGF-1Rinliverandmuscleweredetectedbyimmunohis-tochemist~a
8、ndreversetranscription-polymerasechainreaction.ResultsThelevelsofplasmainsulin,gluca.gon,adrenalin,andcorticosteroidintheIGF-1groupweresignificantlylowerthanthoseinpyaemicgroupandPNgroup(P<0.05,P<0.O1),andIGF一1levelinIGF-1g
