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1、RP-HPLC同时测定鼠掌老鹳草中5种活性成分的含量罗宏，尹海波（辽宁中医药大学药学院，辽宁大连116600）[摘要]目的：建立HPLC法同时测定鼠掌老鹳草中没食子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-а-二鼠李糖苷的含量测定方法。方法：采用RP-HPLC法，AgilentTC-C18色谱柱(250mm*4.6mm)，0.4％磷酸水溶液-甲醇为流动相梯度洗脱，流速0.8mL·min-1,检测波长为切换波长,(0-23min)波长270nm;(23-55min)波长370nm.柱温30℃。结果：没食
2、子酸,原儿茶酸,槲皮素,山奈素,山奈素-3,7-O-α-二鼠李糖苷的线性范围为:0.192~1.732g.L-1(r=0.9997),0.016~0.256g.L-1(r=0.9999),0.004~0.039g.L-1(r=0.9999),0.004~0.041g.L-1(r=0.9999),0.2004~1.6032g.L-1(r=0.9998),平均回收率（n=3）分别为100.07%(RSD1.5%),99.55%(RSD1.7%),100.28%(RSD2.2%),99.99%(RSD1.8%),9
3、9.90%(RSD1.0%)。结论：该含量测定的方法快速、准确、可靠、重复性好，可为药材的质量控制提供依据。【关键词】鼠掌老鹳草；没食子酸；原儿茶酸；槲皮素；山奈素；HPLCSimultaneousdeterminationoffiveactivecompoundsinGeraniumsibiricumL.byRP-HPLCLUOHong,YINHaibo(LiaoningUniversityofTraditionalChineseMedicine，Dalian116600，China)[Abstract]O
4、bjective:Todetermine5activecompoundsinGeraniumsibiricumL.,namelygallicacid,protocatechuinacid,quercetin,kaempferol-7-O-rhamnosideandkaemferitrin.Method:TheRP-HPLCmethodwasappliedandtheseparationwasperformedonanAgilentTC-C18analyticalcolumn(250mmХ4.6mm,5μm).
5、Themobilephasewasmethanol(A)-watercontaining0.4%H3PO4(B)withgradientelutionmodeattheflowrateof0.9mL•min.Thedetectionwassetat270nm(0-23min),370nm(23-55min),andthecolumntemperaturewas30℃.Result:ThelinerrangesofGallicacid,protocatechuinacid,quercetin,kaempfero
6、l-7-O-rhamnosideandkaemferitrinwere0.192~1.732g.L-1(r=0.9997),0.016~0.256g.L-1(r=0.9999),0.004~0.039g.L-1(r=0.9999),0.004~0.041g.L-1(r=0.9999),0.2004~1.6032g.L-1(r=0.9998).Theaveragerecoveries(n=3)were100.07%(RSD1.5%),99.55%(RSD1.7%),100.28%(RSD2.2%),99.99%
7、(RSD1.8%),99.90%(RSD1.0%).Conclusion:ThemethordissimpleandaccuratewithagoodreproducibilityandcanbeusedasaqualitycontrolmethordforGeraniumsibiricumL.ofdifferentsources.[Keywords]GeraniumsibiricumL.;Gallicacid;Protocatechuinacid;Quercetin;Kaempferol-7-O-rhamn
8、oside;HPLC老鹳草为我国传统中药，来源于牻牛儿苗科植物牻牛儿苗（ErodiumstephanianumWilld.）、老鹳草（GeraniumwilfordiiMaxim.）或野老鹳草(GeraniumcarolinianumL.)的干燥地上部分,收载于我国10版药典，前者习称”长嘴老鹳草”，后两者习称”短嘴老鹳草”[1]。性辛、苦、平，归肝、肾、脾经，祛风湿、通经络、主治筋骨酸痛、泄泻痢疾
