spf鸡感染vvibdv后免疫器官内肥大细胞及5-ht动态变化的观察

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1、SPF鸡感染vvIBDV后免疫器官内肥大细胞及5-HT动态变化的观察SPF鸡感染vvlBDV后免疫器官内肥大细胞及5一HT动态变化的观察熊金茂,王德成,余锐萍,罗冬梅,陈丽丽,岳卓中国农业大学动物医学院.北京100094【摘要】为观察肥大细胞(MastCell,MC)在wIBDV感染中的作用,将6O只3O日龄无病原感染(SpecificPathogenFree,SPF)鸡随机分成感染组和对照组.感染组经滴鼻,点眼,肛拭感染超强毒株传染性法氏囊病病毒(veryvirulentInfectiousBursalDiseaseV

2、irus,wIBDV)于O,24,48和72h后,分别取法氏囊,胸腺,脾脏,用甲苯胺蓝染色法观察上述各组织中的MC数量的动态变化;用免疫组化染色法检测各组织中IBDV病毒抗原和5一HT分布量的动态变化.结果表明:与对照组相比.感染组鸡各免疫器官中,MC的数量随着病毒分布量的增加和组织损伤程度的加重而明显增多(P<0.01):MC释放的介质之一——5一HT的量也明显增多(P<0.01).由此推断,MC和5一HT参与了IBD的发病过程.它们在IBDV感染引起的免疫器官严重损伤中可能起着重要的作用.【关t词】肥大细

3、胞;5一HT;lBDV;免疫组化【中圈分类号】$851.34+7-2【文献标识码】A【文章绩号】1000—7857i2007)13—0034—07StudyontheDynamicChangeofMastCelland5-HTinImmuneOrgansofSPFChickensInfectedbyVeryVirulentStrainsofIBDV(vvIBDV)XIONGJinmao,WANGDecheng,SHERuiping,LUODongmei,CHENLili,YUEZhuoCollegeofVeterinar

4、yMedicine,ChinaAgricuhralUniversity,Beijing100094,ChinaAbstract:InordertostudythepotentialroleofmastcellsinSpecialPathogensFree(SPF)chickensinfectedbytheveryvirulentInfectiousBursalDiseaseVirus(vvIBDV),60SPFchickensof30daysofageweEerandomlydividedintotwogroups(co

5、ntrolgroupandvvIBDVtestedgroup).ThechickensinthetestedgroupwereinnoculatedwithvvIBDVthroughnosedrop,eyedropandanusrub.BursaofFabricious,thymusandspleenweretakenat0,24,48,72hpi(hourspost—infection).ThedynamicchangeofmastcellwastestedbyimprovedToluidineBlue(TB)stai

6、ning.ThequantitychangeofvvIBDVviralantigenand5-HTintissuesweretestedbyimmunohistochemistrv.Theresuhsshow:vvIBDVviralantigenisextensivelydistributedinthethymus,spleen,andbursaofFabricious,however,thelocalizationofvvIBDVviralantigenisdifferentindifferenttissuesando

7、rgans.Obviously,thepositivesubstanceofvvIBDVviralantigenintheexperimentalgroupenhancestheinflammatoryreactioninthemajorimmuneorganssuchasbursaofFabricius,spleenandthymus.②Thenumberofmastcellsobviouslyincreasesintheexperimentalgroup(尸<0.01)aftervvIBDVinfection.

8、Comparedwiththecontrolgroup,themastcellsgraduallyincreasefrom24hpito48hpiand72hpi<0.o5).Andfewmastcellscouldbefoundinthebursa,thymusandspleenofcontrolgroup.