rev感染对spf雏鸡外周血液及免疫器官tgfβ1含量变化的影响

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1、CONTENTSCoNTENTSAbstractinI：：hinese．．．．1AbstractinEnglish．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．III1．Introduction11．1ResearchprogressofREV．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．11．1．1MolecularbiologicaIcharacteristicsofREV．11．1．2EpidemiologicalofREV．．．．．．．21．1．3

2、ClinicalsymptomsandpathologicalchangesofRE．．．．．．．．．．．．．．．．．．．．．．．．．．．31．1．4ImmunosuppressionofREV．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．31．1．5DifferentialdiagnosisofRE．．．．．．．．．．．．．．．．．．．．．．．41．1．6PreventionandtreatmentofRE51．2ResearchprogressofTGF-131．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．51．

3、2．1IntriductionofTGF—B1．51．2．2SignalingpathwayofTGF一131．．．．．51．2．3BiologicalfunctionofTGF一131．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．61．3Investigativepurposeandsignificance．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．82．MaterialsandMethods．92．1Experimentalmaterials．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．

4、．．．．．．．．．．．．．．．．．．．．92．1．1Experimentalanimal．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．92．1．2】；也Vstrains．．．92．1．3Mainbiologicalandchemicalreagents．92．1．4Mainequipment．．．102．2Experimentalmethods．．．1()2．2．IExperimentalanimalgroupingandprocessing．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．102．2．2

5、Experimentalmaterialadoptsandprocessing．．．．．．．．．．．．．102．2．3Indexandmethodofdetection．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．112．3Dataprocessing．．．．．．．．．．．．．．．．．．．．．．173．Resultsandalialyrsis．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．

6、．．．．．．．．．．．．．．．．．．．．．．．．．．183．1ChangesoftheexpressionofTGF·131mRNAinperipheralbloodandimmuneorgansofSPFchicksirifectedwithREV．．183．1．1ExtractionoftotalRNA183．1．2PCRidentificationofrecombinantplasmid．193．1．3Drawofstandard．．203．1．4DetectionofexpressionofTGF-p1mRNAandp-actinmRNAinperipheralbloo

7、dofSPFchicksirifectedwithREV223．1．5ChangesoftheexpressionofTGF一131mRNAinperipheralbloodofSPFchicksirifectedwithREV．23III万方数据CONlENIS3．1．6DetectionofexpressionofTGF一131mRNAand13-actinmRNAinimmuneorgansofSPFchicksinfectedwithREV．．243．1．7Changesofthee