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ID:28572363
大小:15.39 MB
页数:57页
时间:2018-12-11
《rev感染对spf雏鸡外周血液及免疫器官tgfβ1含量变化的影响》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、CONTENTSCoNTENTSAbstractinI::hinese....1AbstractinEnglish..............................................III1.Introduction11.1ResearchprogressofREV..............................................................11.1.1MolecularbiologicaIcharacteristicsofREV.11.1.2EpidemiologicalofREV.......21.1.3
2、ClinicalsymptomsandpathologicalchangesofRE...........................31.1.4ImmunosuppressionofREV................................31.1.5DifferentialdiagnosisofRE.......................41.1.6PreventionandtreatmentofRE51.2ResearchprogressofTGF-131.............................................51.
3、2.1IntriductionofTGF—B1.51.2.2SignalingpathwayofTGF一131.....51.2.3BiologicalfunctionofTGF一131...............................61.3Investigativepurposeandsignificance.................................................82.MaterialsandMethods.92.1Experimentalmaterials................................
4、....................92.1.1Experimentalanimal..............................................92.1.2】;也Vstrains...92.1.3Mainbiologicalandchemicalreagents.92.1.4Mainequipment...102.2Experimentalmethods...1()2.2.IExperimentalanimalgroupingandprocessing.......................................102.2.2
5、Experimentalmaterialadoptsandprocessing.............102.2.3Indexandmethodofdetection........................................................................112.3Dataprocessing......................173.Resultsandalialyrsis.......................................................................
6、..........................183.1ChangesoftheexpressionofTGF·131mRNAinperipheralbloodandimmuneorgansofSPFchicksirifectedwithREV..183.1.1ExtractionoftotalRNA183.1.2PCRidentificationofrecombinantplasmid.193.1.3Drawofstandard..203.1.4DetectionofexpressionofTGF-p1mRNAandp-actinmRNAinperipheralbloo
7、dofSPFchicksirifectedwithREV223.1.5ChangesoftheexpressionofTGF一131mRNAinperipheralbloodofSPFchicksirifectedwithREV.23III万方数据CONlENIS3.1.6DetectionofexpressionofTGF一131mRNAand13-actinmRNAinimmuneorgansofSPFchicksinfectedwithREV..243.1.7Changesofthee
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