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1、StudyofcellulolyticsoilfungiandtwonovaspeciesandnewmediumKhalideta1./JZhejiangUnivSCIENCEB20067(6).'459?466JournalofZhejiangUniversitySCIENCEBISSN1673.1581(Print);ISSN1862—1783(Online)v,,v,v.zju.edu.cn/jzus;wwv4.springerlink.comE—mail:jzus@zju.edu.cn4
2、59Studyofcellulolyticsoilfungiandtwonovaspeciesandnewmedium{ALIDMahmoo(It,-,YANGWei-jun,sHwARNazir3,RAJPuTZahidIqbal,ARIJOAbdullahG.fGovernmentCollege,SatelliteTown,Rawalpindi46000,Pakistan)(SchoolofLifeSciences,ZhejiangUniversity,Hangzhou310058,China
3、)(JNaturalHistoryMuseum,Islamabad44000,Pakistan)f&DSection,DirectorateofAnimalHusbandrySindh,ShahbazBuilding,Hyderabad71000,Pakistan)(DepartmentofParasitology,SindhAgricultureUniversity,TandoJam70060,Pakistan)E-mail:kmahdi2005@yahoo.comReceivedMar
4、.17,2006;revisionacceptedApr.14,2006Abstract:Thisstudyisaimedatidentilyinganddeterminingthepercentageofoccurrencefrequencyofcellulosedecomposingsoilfungi刀,esoilsampleswereinoculatedintocultureplatespreparedinSabouraudmediUlTIundersterilizedconditionsa
5、ndincubatedat30.Cfor4to7d.Theidentifiedfungalspecieswereincubatedinself-designedcellulosemediumfortestingtheircellulolyticability.Forty-twospecies,including.2novaspecies,representingsixteengenerashowedgrowthandsporulationinthecellulosemedium.Mostofthe
6、isolatedspecieswerefromgenusAspergillusandPenicillium.AspergillusnigerandMucorhie.showedhighestoccurrencefrequency(45%and36%respectively).asthesespecieswerecollectedfromabout80%ofsoilsamples.Beingagarfreeandcheaper,thenewfungalmediumdesignedshowedresu
7、ltsequivalenttoSabouraudmedium.Keywords:Cellulolyticsoilfungi,Cellulosemedium,Novaspecies,Taxonomydoi:10.1631/jzus.2006.B0459Documentcode:ACLCnumber:Q949.3INTRODUCTIoNStudyoflungifromthetaxonomicpointofviewisofpracticalvalueandtheimportanceoftaxonomyi
8、sevidentfromapaperwhoseauthormentioned20cellulolyticspeciesofgenusCladosporiumbyhum.berinsteadofname(Ghahfarokhieta1..2004).Apartfromtheirdiseasecausingnature(Wainwright,l995),theirbeneficialeffectsandincreasingroleinthein.dustrialarea(Anderet
