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1、治疗乙型肝炎新药肝龙胶囊的药效学初步研究论文杜一民,陈鸿珊,李树楠,李壮,李小兵,张华明,方春生【关键词】肝龙胶囊;,,鸭乙型肝炎病毒;,,鸭乙肝动物模型;,,实验性肝损伤;,,美洲大蠊摘要:目的观察肝龙胶囊对鸭乙型肝炎病毒的抑制效果和对实验性肝损伤的保护作用。方法(1)以鸭乙型肝炎病毒(DHBV)静脉注射感染雏鸭为模型,于感染第7天后分组灌胃肝龙(GL)胶囊浸膏0.5.freelgkg1)。测定SGPT值,并取肝脏作病理形态学观察。结果(1)GL三个不同剂量组对DHBV均有不同程度的抑制作用,有一定的量效关系;(2)GL降低C
2、Cl4肝损伤小鼠血清SGPT值并减轻肝细胞损伤。结论肝龙胶囊可抑制雏鸭体内DHBVDNA的复制并能保护CCl4所致的小鼠肝损伤。关键词:肝龙胶囊;鸭乙型肝炎病毒;鸭乙肝动物模型;实验性肝损伤;美洲大蠊TheHepaticPharmacologicalEffectsofGanlongCapsulesinvivo.Abstract:ObjectiveToobservetheantiviralactivityofGanlongCapsuleagainstduckHepatitisBvirus(DHBV)anditsprotective
3、effectsonexperimentalliverinjuryinvivo.Methods(1)ExperimentalresearchesonantiviralactivityofGanlongCapsulesagainstduckhepatitisBvirus:TheduckHepatitisBmodeladebyinjectingDHBVintotheveinsofone-day-oldBeijingduckontheseventhdayafterinfectedbyDHBV,GanlongCapsuleinistrat
4、ionfor10daystogroupI(0.5gkg1d1),groupII(1.5gkg1d1),groupIII(3.0gkg1d1),bid×10d,.freeldotblothybridization.Thetrialate(PFA).(2)ProtectiveeffectsofGanlongcapsuleonexperimentalliverinjuryinmice:Acutehepaticinjurylkg1,Ganlong(200mgkg1)inistrationfor2days,bid.TheserumSGPT
5、inedandthehistopathologicalchangesofhepatictissueeasured.Results(1)TheresultsofthreeexperimentsshoentgroupsofGanlong,andinadose-effectmanner.ThegroupIII(3.0gkg1d1)inhibited(P0.01)theserumDHBVDNAsignificantly,andafterstoppingthetreatmentfor6days,serumDHBV-DNAleveldidn
6、otreturnsignificantly,andtheinhibiteffectsentgroupofphosphonoformate(PFA)oracyclovor(ACV).(2)Ganlong(200mgkg1)couldobviouslyinhibittheincreaseofserumSGPT,andhistologicalexaminationshoagesofGanlongtreatedmicearkedlyinhibitedduckhepatitisvirusreplicationandhadprotectiv
7、eeffectsonexperimentalhepaticinjuryinvivo.Keyalhepatitismodel;Experimentalhepaticinjury;PeriplaaamericanaL.我国是乙肝高发国之一,乙型肝炎感染率高、危害严重。目前,用于治疗乙肝的药物有数百种,但仍缺乏理想的防治药物。云南大理学院研制的治疗乙肝的新药肝龙胶囊,是国家实行新药研究开发登记备案制以来,国家食品药品监督管理局正式登记在案的抗肝炎中药新药[1],具有疗效较好、价廉、给药方便、无副作用等优点[2],目前已经完成临床试验,
8、正在申请试生产。该药由提取自昆虫美洲大蠊PeriplaaamericanaL.的活性成分研制,对实验性肝损伤有一定的保护作用,并能抑制雏鸭体内鸭乙型肝炎病毒的复制。现将研究结果报道如下。1材料1.1药物肝龙胶囊和肝龙浸膏由云南大理医学院药理教研室研制,浸膏干粉用