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1、PlantGenomicsMethodsandProtocolsEditedbyDarylJ.Somers*,PeterLangridge†,andJ.PerryGustafson‡MolecularBreedingandBiotechnology,VinelandResearchandInnovationCentre,VinelandStation,Ontario,Canada*AustralianCentreforPlantFunctionalGenomics,UniversityofAdelaide,GlenOsmond,
2、Australia†DivisionofPlantSciences,UniversityofMissouri,Columbia,MO,USA‡PrefaceThisvolumeisdividedintochapterswhichconsidertheprimaryissuesandmethodologiessurroundingplantgenomicsresearch.Plantgenomicsislargelyconcernedwithassociatingfunctionalgenesorgenemutationswith
3、phenotype.Therefore,chaptersareincludedthatcovertheareasofgenediscoveryandfunctionalanalysisofgenes.Furtherchaptersfocusontheprimarytoolsandsub-disciplinesofgeneticmapping,mRNA,proteinandmetaboliteprofiling.Methodsareincludedthatexploregenefunctionalanalysisviatransf
4、ormation,mutation,proteinfunctionandgeneexpression.Thevolumeincludeschaptersondatamanagementwhichconsidertheexpansionofplantgenomicsdatabasesandbioinformaticsanalysistools.Thevolumeisconcludedwithchaptersaimedatdiscussingtheapplicationanddeploymentofmolecularplantbre
5、edingtechnologyfromtheuseofmarkersinbreeding,developmentofgeneticallymodifiedplants/cropspecies,analysisofexistingpopulationsfornovelallelesandgene/traitassociationsandgenomesequencing.ContentsPreface............................................................vContri
6、butors........................................................ix1RoleofModelPlantSpecies.........................................1RichardFlavell2NewTechnologiesforUltra-HighThroughputGenotypinginPlants..........19NikkiAppleby,DavidEdwards,andJacquelineBatley3GeneticM
7、apsandtheUseofSynteny..................................41ChrisDuran,DavidEdwards,andJacquelineBatley4ASimpleTAE-BasedMethodtoGenerateLargeInsertBACLibrariesfromPlantSpecies.....................................57Bu-JunShi,J.PerryGustafson,andPeterLangridge5TranscriptP
8、rofilingandExpressionLevelMapping........................81ElenaPotokina,ArnisDruka,andMichaelJ.Kearsey6MethodsforFunctionalProteom