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1、中国水稻科学(ChinJRiceSci),２０１４,２８(６):５５９－５６９http://www．ricesci．cnDOI:１０．３９６９/ji．ssn．１００１Ｇ７２１６．２０１４．０６．００１５５９一个抗病性增强的水稻类病变突变体的蛋白质组学研究韩雪颖１,２杨勇２余初浪２张文浩３叶胜海２陈斌２程晨２程晔２严成其２陈剑平１,２,∗(１南京农业大学植物保护学院,南京２１００９５;２浙江省植物有害生物防控重点实验室———省部共建国家重点实验室培育基地/农业部植保生物技术重点实验室/浙江省植物病毒学重点实验室/浙江省农业科学院病毒学与生物技术研究所,杭州３
2、１００２１;３中国计量学院生命科学学院,杭州３１００１８;∗通讯联系人,EＧmail:jpchen２００１＠１２６．com)AProteomicStudyonaDiseaseＧResistanceＧEnhancedRiceLesionMimicMutantHANXueＧying１,２,YANGYong２,YUChuＧlang２,ZHANGWenＧhao３,YEShengＧhai２,CHENBin２,CHENGChen２,CHENGYe２,YANChengＧqi２,CHENJianＧping１,２,∗(１CollegeofPlantProtection,Na
3、njingAgriculturalUniversity,Nanjing２１００９５,China;２StateKeyLaboratoryBreedingBaseforZhejiangSustainablePestandDiseaseControl,KeyLaboratoryofBiotechnologyinPlantProtection,MOA,ZhejiangProvincialKeyLaboratoryofPlantVirology,InstituteofVirologyandBiotechnology,ZhejiangAcademyofAgricult
4、uralScience,Hangzhou３１００２１,China;３CollegeofLifeScience,ChinaJiliangUniversity,Hangzhou３１００１８,China;∗Correspondingauthor,EＧmail:jpchen２００１＠１２６c．om)HANXueying,YANGYong,YUChulang,etal．AproteomicstudyonadiseaseＧresistanceＧenhancedricelesionmimicmutant．ChinJRiceSci,２０１４,２８(６):５５９Ｇ５６９．A
5、bstract:AlesionＧmimicmutantinrice(OryzasativaL．),chloroplasticＧH２O２Ｇinducedlesion１(chl１),hasenhancedresistancetoriceblastandbacterialblight．Tounderstandthemolecularmechanismsunderlyingthisphenotypeanditsresistanceresponses,aproteomicsＧbasedapproachwasusedtoidentifydifferentiallyＧe
6、xpressedproteinsbetweenchl１anditswildtype．UsingtwoＧdimensionalfluorescencedifferencegelelectrophoresistechnologyandmassspectrometry,７０proteinspotsweresuccessfullyidentified,ofwhich４６wereupＧregulatedand２４weredownＧregulatedinthemutant．ThesedifferentiallyＧexpressedproteinsareinvolved
7、indiversebiologicalprocessesincludingdiseaseresistance,photoＧsynthesis,oxidationＧreductionreaction,aminoacid/proteinmetabolism,chaperoningandcarbohydratemetabolism．ThecomplexregulatorynetworkinwhichtheseproteinsareinvolvedmayplayanimportantroleinregulatingtheproＧgrammedcelldeathan
8、dtheresistancereactioninchl１．Keyw
