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1、枯草芽孢杆菌作为新的益生菌演讲稿INTRODUCTIONProbiotics:livemicroorganismspositiveeffectondiversefunctionsofanorganismpreventtheinvasionofvariouspathogensExperimentswithmiceprovedthatB.subtilissporeswereabletogerminate,andthecellscouldproliferateandformthesporesagaininintestinesof
2、animalsAutochthonouslacticacidbacteriaofthegeneraLactobacillusandBifidobacteriumareusuallyusedasprobiotics;grampositivespore-formingbacteriaofthegenusBacillusareusedasprobioticsaswellStudieshaverevealedthatinadditiontotheresistanceoftheprobioticBacillusstrainstobi
3、leacids,theywerecapableofimmunestimulationincaseofgastrointestinaldisorders.Abilitytosynthesizeantibiotics,bacteriocins,cycliclipopeptides,andlyticenzymeswithantimicrobialactivityprovidesprobioticactivityforbacteriaofthegenusBacillus.Beingprobiotics,thestrainsofB.
4、subtilisandB.coagulanswereshowntohaveagrowth-stimulatingandprophylacticeffectonbroilerchickens1INTRODUCTION标题Thegoalofthepresentworkwastocharacterizethepropertiesofthesenewstrainsandtoassesstheirpotentialuseasprobiotics.01INTRODUCTIONMATERIALSANDMETHODSThesubjects
5、ofthestudywereB.subtilisstrainsGM2andGM5withhighantimicrobialactivity(Mardanovaetal.,2017).Opportunisticcoliformbacteria,SalmonellaentericaserotypeTyphimuriumATCC14028s,Klebsiellaoxytoca,andEscherichiacoli,aswellasmicromycetesFusariumavenaceum,F.oxysporum,F.redole
6、ns,andF.solani,wereusedastestcultures.02MATERIALSANDMETHODSNutrientmediaandcultivationconditions.Thefollowingmediawereusedforcultivation.Thesewere: (1)LB(Luria–Bertoni)medium(g/L):tryptone,10.0;yeastextract,5.0;NaCl,5.0;(2)LAmedium(g/L):tryptone,10.0;yeastextract,
7、5.0;NaCl,5.0; agar,20.0;(3)mediumno.1(g/L):maltose,20.0;peptone,10.0;CaCl2,1.0;(4)mediumno.2(g/L):soybeanmeal,30.0;NaNO3,3.0;K2HPO4,1.0;MgSO4,0.2;KCl,0.2;(5)mediumno.3(g/L):cornextract,20.0;lactose,10.0;(6)deoxycholatecitrateagarforsalmonellacultivation.Bacteriawe
8、recultivatedinathermostatat37°CandusinganIKA®KS4000incubatorshaker(Germany)at37°Candrotationspeedof200rpm.OpticaldensityoftheculturewasmeasuredusingaBio
