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1、NewBacillussubtilisStrainsasPromisingProbioticsFRONTIERSINIMMUNOLOGYCONTENTS01020304INTRODUCTIONMATERIALSANDMETHODSRESULTSANDDISCUSSIONCONCLUSIONINTRODUCTIONProbiotics:livemicroorganismspositiveeffectondiversefunctionsofanorganismpreventtheinvasionofvariouspathogensExperimentswi
2、thmiceprovedthatB.subtilissporeswereabletogerminate,andthecellscouldproliferateandformthesporesagaininintestinesofanimalsAutochthonouslacticacidbacteriaofthegeneraLactobacillusandBifidobacteriumareusuallyusedasprobiotics;grampositivespore-formingbacteriaofthegenusBacillusareused
3、asprobioticsaswellStudieshaverevealedthatinadditiontotheresistanceoftheprobioticBacillusstrainstobileacids,theywerecapableofimmunestimulationincaseofgastrointestinaldisorders.Abilitytosynthesizeantibiotics,bacteriocins,cycliclipopeptides,andlyticenzymeswithantimicrobialactivityp
4、rovidesprobioticactivityforbacteriaofthegenusBacillus.Beingprobiotics,thestrainsofB.subtilisandB.coagulanswereshowntohaveagrowth-stimulatingandprophylacticeffectonbroilerchickens1INTRODUCTION标题Thegoalofthepresentworkwastocharacterizethepropertiesofthesenewstrainsandtoassesstheir
5、potentialuseasprobiotics.01INTRODUCTIONMATERIALSANDMETHODSThesubjectsofthestudywereB.subtilisstrainsGM2andGM5withhighantimicrobialactivity(Mardanovaetal.,2017).Opportunisticcoliformbacteria,SalmonellaentericaserotypeTyphimuriumATCC14028s,Klebsiellaoxytoca,andEscherichiacoli,aswe
6、llasmicromycetesFusariumavenaceum,F.oxysporum,F.redolens,andF.solani,wereusedastestcultures.02MATERIALSANDMETHODSNutrientmediaandcultivationconditions.Thefollowingmediawereusedforcultivation.Thesewere:(1)LB(Luria–Bertoni)medium(g/L):tryptone,10.0;yeastextract,5.0;NaCl,5.0;(2)LAm
7、edium(g/L):tryptone,10.0;yeastextract,5.0;NaCl,5.0; agar,20.0;(3)mediumno.1(g/L):maltose,20.0;peptone,10.0;CaCl2,1.0;(4)mediumno.2(g/L):soybeanmeal,30.0;NaNO3,3.0;K2HPO4,1.0;MgSO4,0.2;KCl,0.2;(5)mediumno.3(g/L):cornextract,20.0;lactose,10.0;(6)deoxycholatecitrateagarforsalmonell
8、acultivation.Bacteriawerecultivatedinathermosta
