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ID:5750742
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时间:2017-12-24
《raman tag imaging technology拉曼标记技术》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、Ramantagimagingtechnology Ramanspectroscopycanbeusedtodetectsmallmolecules bydetectingtheirmolecularvibrations.However, forobservationincells,Ramanscatteringfromsmall moleculesareoftenoverlappedwiththosefromendogenous molecules,andcannoteasilybeextracted. Toovercome th
2、isproblem,theso-calledRamantagtechnique hasbeendevelopedtoseparatethetargetmoleculesfrom others. Ramantagimagingtakesadvantageof thesilentregionintheRamanspectraofcells.Thesilentregionexistsbetween1800and2800cm_1wheremostbiologicalmoleculesdonotproduceRamanscatteringsi
3、gnals.Fortunately,functionalgroups,suchasalkyne,azide,deuterium,andnitrile,showcharacteristicRamanbandsinthesilentregion.Thus,thesesubstituentscanbeusedastagsforobservationofsmallmoleculesusingRamanscattering.Ref: Palonpon,A.F.,etal.,2013.
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