欢迎来到天天文库
浏览记录
ID:56168930
大小:603.67 KB
页数:4页
时间:2020-06-04
《麦秸饲料转化中木质纤维素软化技术研究.pdf》由会员上传分享,免费在线阅读,更多相关内容在应用文档-天天文库。
1、l28广东农业科学2014年第13期麦秸饲料转化中木质纤维素软化技术研究李传友,何润兵,崔宗均(1.北京市农业机械试验鉴定推广站,北京100079;2.北京市大兴区农业机械技术推广站,北京102612;3.中国农业大学农学与生物技术学院,北京100183)摘要:为解决秸秆饲料转化中消化率低的问题,开发秸秆分解软化技术,优先分解部分半纤维素,以小麦秸秆为原料,以半纤维素分解为特色的分解菌复合系为接种剂,对麦秸进行高温发酵分解,通过检测秸秆木质纤维素各成分的动态、发酵参数变化,以及PCR—DGGE为手段的微生物群动态监测,研究秸秆木质纤维素结构松散的规律。结果表明,经过高温发酵
2、,秸秆中半纤维素显著降解,9d时半纤维素和纤维素分别分解了11.2%和9.3%;在高温发酵过程中,微生物群以细菌为主体,真菌种类较少,且细菌群中以接入的分解菌复合系的组成菌占优势。说明向高温发酵体系中添加分解菌复合系可以有效促进发酵进程,使麦秸在发酵第9d时半纤维素的降解达到要求,有效地松散了秸秆结构。关键词:秸秆饲料;半纤维素分解;微生物群动态中图分类号:S816.9文献标识码:A文章编号:1004—874X(2014)13—0128—05ResearchonsofteninglignocellulosetechnologyfIoOrconvertilng~wWh11ea
3、tstrawintofIeedLIChuan-you,HERun—bing,CUNZong-jun(1.BeijingAgriculturalMachineryTestingExtensionSt~ion,Beijing100079,China;2.BeijingDaxingDistrictAgriculturalMachineryTechnologyExtensionSt~ion,Beijing102612,China;3.CollegeofAgronomyandBiotechnology,ChinaAgriculturalUniversity,Beijing100183
4、,China)Abstract:Toimprovethedigestibilityduringfeedconversationofstraw,thisstudyinvestedatechnologyaboutdegradingfirstlypartialhemicellulosetosoftenwheatstrawduringfermentationinvitro.Fermentationwasconductedathightemperaturewithastartercultureofamicrobialcommunitywiththehighcapabilityofde
5、gradinghemicellulose.Theruleofincompactconfigurationoflignocellulosewasdetectedthroughtracingthedynamicchangesoflignocellulosecomponents,fermentationsparametersandmicrobialdynamicsusingPCR-basedDenaturingGradientGelElectrophoresis(PCR-DGGE).Theresultshowedthatafter9doffermentation,thedegra
6、dationofhemicellulosewassignificant,andthedegradationratioofhemicelluloseandcellulosewerel1.2%and9.3%.respectively.TheprofilesofPCR—DGGEshowedthatthebacteriawerepredominantduringthefermentation.Thespeciesoffungiwereless.Amongthebacterialspeciesdetected,thoseofthecommunitywereoverwhelming.T
7、hisshowedthatinoculatingthemicrobialcommunityintothefermentationsystemcouldsignificantlyacceleratethefermentationprocess.After9doffermentation,thehemicellulosewaswelldegradedandthestructureofthestrawwaseffectivelyloosed.Keywords:strawfeed;hemicellulosedegradat
此文档下载收益归作者所有