曲美他嗪与雷米普利对慢性心力衰竭大鼠心肌组织转化生长因子β1表达的影响.pdf

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1、中华老年心脑【札臀病杂志20li年J月第16卷第l剃ChinjC,efiatr}teartBrainVesselDis，Jan20l4．Vd16．No．1心力衰竭研究．曲美他嗪与雷米普利对慢性心力衰竭大鼠心肌组织转化生长因子』3表达的影响彭玉娟，谢亚芹，康大伟，冯翔宇，李秀华摘要：目的探讨曲美他嗪和雷米普利单用及联合应用对慢性心力衰竭大鼠左心室心肌组织转化生长因子8(r(F-)的影响。方法利用肾上腹主动脉缩窄法制作雄性Wistar大鼠慢性心力衰竭模型，随机分为模型组、曲美他嗪组、雷米普利组、联合用药

2、组和假手术组，每纽】0只。观察各组大鼠左心室重量指数(LVM1)和左心室舒张末压(IVEDP)、左心室压力最大上升及下降速率(±dp／dt)，采用Massion染色法光镜下观察左心室心肌形态结构变化，sI免疫组织化学法检测各组大鼠左心室心肌中TGF一8表达情况，RT～PCR测定各组大鼠左心宣心肌TGF～口mRNA水平。结票与模型组比较．曲美他嗪组、雷米普利组和联合用药组土dp／dt明显升高．LVEI)P、IVMI、TGF—p阳性表达和T(；F～BmRNA水平明显降低，联合用药组改变更为显著。光镜下显

3、示，曲美他嗪组、雷米普利组及联合用药组心肌损害程度较模型组明显减轻，联合用药组改变更为显著。结论曲美他嗪与雷米普利单用及联合应用均可有效改善心功能、减轻心肌纤维化，其中联合用药优于单独用药。关键词：心力衰竭；曲美他嗪；雷米普利；转化生长因子81；逆转录聚合酶链反应EffectoftrimetazidineandramiprilonexpressionofTGF—plinmyocardialtissueofchronicheartfailureratsPENOYu—juan，XIEYa—qin，KAN

4、(；Da—wei，etal(Department0，’Geria~’，，(s，A，／iliatedHospitalo，(、^engdeMedicⅥCollege．．Chengde067000，HebeiProvince，(：hina)Abstract：0bjectiveI'ostudytheeffectoftrimetazidineandramiprilonexpressionofTGF—l31inmyocardMtissueofchronicheartfailure(CHF)rats．Method

5、sACHFmodelofWistarratswasestabablishedbyconstrictingthesuprarenalabdominalaorta．FiftyWistarratswererandomlydi—videdintomodelgroup，trimetazidinetreatmentgroup，ramipriltreatmentgroup，cornbinedtreat—mentgrouPandshamoperationgroup(10ineachgroup)．Theleftven

6、tricularmassindexes(I．VMI)，leftventricularenddiastolicpressure(I．VEDt)andmaximumleftventricularpressurerisingandfalling(士dp／dt)weremeasured．MyocardialmorpholyofleftventriclewasobservedunderopticalmicroscopewithMassonstaining．ExpressionofTGF一andTGF—pltu

7、RNAinleftventriclewasdetectedbySPimmunohistochemistryandRT—PCR，respectively．ResultsThe±dp／dtwassignificantlyhigher，whereastheI．VEDPandLVMI，theexpressionlevelofTGF-~】andTOF—f3ImRNAweresignificantlylowerintrimetazidinetreatmentgroup，ramipriltreatmentgrou

8、pandcombinedtreatmentgroupthaninmodelgroup．()pticalmicroscopyshowedthatthemyocardMdamagewaslessintrimetazidinetreatmentgroup，ramipriltreatmentgroupandcom—binedtreatmentgroupthaninmodelgroup．ConclusionTrimetazidineorramiprilaloneandtheir