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1、作物学报ACTAAGRONOMICASINICA2008,34(7):1188−1192http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2008.01188利用原位杂交及CAPS标记分析芸薹属A、B和C基因组间的关系*孔芳蒋金金吴磊王幼平(扬州大学生物科学与技术学院,江苏扬州225009)摘要:以来源于Brassicarapa基因组(AA)的重复序列(151bp)为探
2、针,分别同二倍体白菜型油菜(AA,2n=20)、甘蓝(CC,2n=18)和异源四倍体芥菜型油菜(AABB,2n=36)的中期染色体杂交,白菜型油菜和甘蓝的所有染色体上都有杂交信号,芥菜型油菜的染色体上显示出20个明显的信号,其余染色体上信号很弱或无,可以区分出A和B基因组。对来源于油菜3个基本种与3个复合种FAE1基因进行CAPS分析表明,3个基本种表现出不同的酶切式样,用MboI和MspI酶切表现出多态性,基因组A和C非常相似,而基因组B与A、C关系较远,同时3个复合种也并不是2个基本种的简单相加,表明异源四倍体在长期进化过程中可能
3、发生了重排和重组。关键词:芸薹属;荧光原位杂交;重复序列;CAPS标记;亲缘关系RelationshipsamongGenomeA,B,andCRevealedbyFISHandCAPS*KONGFang,JIANGJin-Jin,WULei,andWANGYou-Ping(CollegeofBioscienceandBiotechnology,YangzhouUniversity,Yangzhou225009,Jiangsu,China)Abstract:PhysicallocalizationofrepetitiveDNAsequ
4、encefromgenomeA(151bp)wascarriedoutonthechromosomesoftheselectedBrassicaspeciesbyFISH(fluorescenceinsituhybridization).ThesignalsdistributedonallthechromosomesofA(Brassicarapa,2n=20)orCgenome(B.oleracea,2n=18).ForB.juncea(AABB,2n=36),thesignalswerefoundonallthechromosom
5、esofgenomeAandthestrengthofsignalvariedamongdifferentchromosomes,whilethechromosomesofgenomeBshowedweakornosignals.FAE1geneisarate-limitinggeneforerucicacidsynthesisinBrassica.ThegenesfromsixBrassicaspeciesofU-triangulewereclonedbyPCR.ThesePCRproductsweredigestedwithdif
6、ferentrestrictionendonucleases.MboIandMspIwerefoundtoproduceinformativeCAPSpatternsofFAE1gene.Threediploidsdisplayeddifferentpatterns,thepat-ternsofgenomeAwasverysimilartothatofgenomeC,whilethepatternsofgenomeBwasthemostdivergedoutofthepatternsoftheAandCgenomes.Threeamp
7、hidiploidsgenerallyexhibitedadditivepatternsoftheprogenitors,butnotstrictlyinallcases,indicatingthatrearrangementsandrecombinationsdidoccurintheformationandevolutionofamphidiploids.Geneticrelation-shipsamongBrassicaspeciescouldbedemonstratedthroughCAPSanalysisofFAE1gene
8、andFISHmethodwhenrepetitiveDNAsequence(notribosomalRNAgenes)wasusedasaprobe.Keywords:Brassica;Fluorescenceinsi
