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时间:2020-04-16
《苦豆子和披针叶黄华种子硬实特性与活力关系研究-论文.pdf》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、西北植物学报,2013,33(8):1665—1673ActaBot.Borea1.-Occident.Sin.文章编号:lOOO一4025(2013)08—1665—09苦豆子和披针叶黄华种子硬实特性与活力关系研究王进,范惠玲,张新慧,谢全刚,张(1河西学院农业与生物技术学院,甘肃张掖734000;2宁夏医科大学药学院,银川750004)摘要:以苦豆子和披针叶黄华种子为实验材料,在恒温25℃下吸胀,每24h取出吸胀种子,10d后未吸胀的种子为硬实种子(H),硬实种子用硫酸处理后恒温吸胀24h,与非硬实种子进行发芽试验和各项活力指标测定。结果显示:(1)每日内吸胀的种子数量
2、随时间推移以一定比列下降,苦豆子非硬实种子吸胀速率较缓慢,第1o天后吸胀率降到1,披针叶黄华非硬实种子第3天后下降到1,第9、10天突然上升后又下降到1。(2)两种豆类种子都显示出硬实种子发芽率、发芽指数、活力指数、脱氢酶、呼吸速率、超氧化物歧化酶(SOD)均高于非硬实种子,而电导率、浸出液可溶性糖和丙二醛(MDA)均低于非硬实种子,缓慢吸胀的硬实种子活力指标高于快速吸胀的硬实种子。研究表明,硬实种子活力高于非硬实种子,硬实种子吸胀过程中存在吸胀损伤。而在非硬实种子中,根据以上活力指标判断,晚吸胀的种子比早吸胀的种子活力高。关键词:苦豆子;披针叶黄华;硬实种子;非硬实种子;
3、种子活力;吸胀损伤中图分类号:Q945.34文献标志码:AStudyonRelationshipbetweenHardseedednessandSeedVigorinSophora口ZDpPcHrDZdandThermopsislanceolataWANGJin,FANHuiling“,ZHANGXinhui。,XIEQuangang,ZHANGYong(1CollegeofAgricultureandBiotechnology,HexiUniversity,Zhangye,Gansu734000,China;2CollegeofPharmacology。NingxiaMe
4、dicalUniversity,Yinchuan750004,China)Abstract:UsingSophoraalopecuroidesandThermopsislanceolataasmaterials,under25℃,theimbibedseedsweretakenoutasasampleevery24h.Thenon—imbibingseedsafter10dwereregardedashardseed(H).Imbibingseedsandhardseedsthatimbibedfor24haftertreatedwithsulfuricacidwereal
5、lsubiectedtogerminationtestandseveralvigourtests.Theresultsindicatedthat:(1)Thenumbersoftheseedsnewlyimbibedinonedayweregraduallydecreasedalongwithtime.ThehardseedsofS.以Z0c“r0已hadarela—tiveslowrateofimbibition,whichwasdecreasedbelow1after10d,whiletheimbibitionrateofT.1an—ceolatawasatthesam
6、eleve1after3d.anotherdecreasehappenedafterasuddenincreasein9thto10thday.(2)Thegerminationrate,germinationindex,vigourindex,theactivityofdehydrogenase,respirationrateandSODactivityofhardseedsweresignificantlyhigherthanthoseofnon—hardseedsinbothspecies,whiletherelativeconductivity,thecontent
7、sofsolublesugarandma1ondialdehydeofhardseedswere1owerthanthoseofnon—hardseeds.Studiesshowedthatthevigourofhardseedswerehigherthanthatofimbi—bingseeds,andthevigourofimbibingseedswereincreasedgraduallywiththeinitialimbibingtimepost一收稿日期:201~,一05一O2;修改稿收到日期2O13—0
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