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1、p16和bcl22基因在膀胱癌中的表达及意义1231何杰,汪万英,朱应葆,姚敏(1.蚌埠医学院病理教研室,安徽蚌埠233003;21蚌埠医学院附属医院,安徽蚌埠233004;31北京大学第三医院,北京100000)摘要:[目的]探讨p16抑癌基因和bcl22凋亡抑制基因与膀胱癌生物学行为的关系。[方法]采用免疫组化和核酸原位杂交等方法检测67例膀胱癌中p16和bcl22基因的表达。[结果]67例膀胱癌p16蛋白表达阳性率52123%(35/67),失表达率47176%(32/67),其中Ⅰ级、Ⅱ级、Ⅲ级的肿瘤p16蛋白失表达率各为14155%、60153%、71143%(P<0105);p16
2、mRNA失表达率分别为20%、40%、80%(P<0105)。而bcl22蛋白表达阳性率59170%(40/67),分别为Ⅰ级36136%、Ⅱ级65179%、Ⅲ级100%,bcl22mRNA阳性表达率各为40%、60%、100%(P<0105)。免疫组化和原位杂交结果一致(P>0105)。[结论]p16基因表达与细胞分化呈负相关,bcl22基因表达与细胞分化呈正相关,提示p16基因的缺失、突变和高甲基化,bcl22基因的异常表达在膀胱癌的发生和发展中起重要的推动作用,两者均可作为判断膀胱癌生物学行为的参考指标。关键词:膀胱肿瘤;基因,p16;基因,bcl22;原位杂交;免疫组化中图分类号:R7
3、37114文献标识码:A文章编号:1671-170X(2001)02-0081-03p16TumorSuppressorGeneandbcl22GeneinBladderTransitionalCellCarcinomaandtheirSignificance123HEJie,WANGWan2ying,ZHUYing2bao,etal.(11DepartmentofPathology,BengbuMedicalCollege,Bengbu233003,China;21TheAffiliatedHospitalofBengbuMedicalCollege,Bengbu233004,China;3
4、1TheThirdAffiliatedHospitalofBeijingUniversity,Beijing100000,China)Abstract:[Purpose]Toevaluatetherelationshipbetweenalterationsofp16tumorsuppressorgene,bcl22apoptosissuppressorgene,andthebiologicalbehaviorsofbladdertransitionalcellcarcinoma(BTCC).[Methods]Sixty-sevencaseswithBTCCwereexaminedforp16a
5、ndbcl22proteinexpressionwithimmunohistochemicalstaining.TwentycasesofBTCCwereexaminedforp16andbcl22mRNAexpressionwithnucleicacidhy2bridizationinsitu.[Results]Thepositiverateofp16proteinexpressionwas52123%(35/67)inBTCC.TherateingradeⅠ,ⅡandⅢwere14155%,60153%and71143%(P<0105),andtheirnegativerateofmRNA
6、expressionwere20%,40%and80%(P<0105)respectively.Thetotalpositiverateofbcl22proteinexpressionwas59170%(40/67),gradeⅠ,ⅡandⅢwere36136%,65179%and100%(P<0105),andtheirpositiverateofmRNAexpressionwere40%,60%and100%(P<0105)respectively.Theresultsofhybridizationinsituwerehighlyconcidentwithimmunohistochemic
7、alstaining(P<0105).[Conclusion]p16geneexpressionisnegativecorrelatedwithcelldifferentiation,whilebcl22geneex2pressionispositivecorrelated.Itisshownthatdeletion,mutationandhypermethylationofp16geneanda
