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1、ArchVirol(1989)107:323-328VrologyArchives©bySpringer-Verlag1989Nucleotidesequenceofthegeneencodingthemembraneproteinofhumancoronavirus229EBriefReportT.RaabeandS.G.SiddellInstituteofVirology,UniversityofWiirzburg,W/irzburg,FederalRepublicofGermanyAcceptedJune22,1989Summar
2、y.Thesequenceofthegeneencodingthemembraneproteinofhumancoronavirus229E(HCV229E)hasbeendetermined.Theprimarytranslationproduct,deducedfromtheDNAsequence,isapolypeptideof225aminoacidswithapredictedmolecularweightof26,000.Thepolypeptidehas3potentialN-glycosylationsites.Many
3、structuralsimilaritieswiththemembraneproteinsofothercoronavirusescanberecognized.ThecoronavirusesareagroupofpositivestrandRNAvirusesthatcauseawidespectrumofdiseaseinmammalsandbirds[21].Thehumancoronavirusesarethoughtocause10-20%ofallcommoncolds,andabouthalfoftheseareasso
4、ciatedwiththehumancoronavirusstrainHCV229E[4,5].TheHCV229EvirioniscomprisedofagenomicRNAofapproximately6x106molecularweight(mol.wt),alipidenvelopeandthreemajorproteins;aphosphorylatedproteinof50,000mol.wt.associatedwiththegenome,aglycosylated180,000mol.wt,proteinandafami
5、lyofpolypeptideswithestimatedmolecularweightsof25,000,23,000,and21,000[7,16].Itisclearthattheseproteinsrepresentthenucleocapsid(N),surface(S)andmembrane(M)proteinschar-acteristicofcoronaviruses[reviewedin19].ThereplicationofHCV229Eappearstofollowthepatternwhichhasnowbeen
6、wellestablishedforseveralcoronaviruses,notably,avianinfectiousbron-chitisvirus(IBV)andmurinehepatitisvirus(MHV)[reviewedin19].IntheHCV229Einfectedcellasetof6T-coterminalsubgenomicmRNAsaresyn-thesized,thesmallestofwhich,mRNA7,encodesthenucleocapsidprotein(22,17].Asforothe
7、rcoronaviruses,thesynthesisoftheHCV229EsubgenomicmRNAsappearstoinvolvealeader-primedmechanismofdiscontinuoustran-324T.RaabeandS.G.Siddellscriptioninwhichaspecificintergenicsequence(TCTAAACforMHVandHCV)playsanimportantrole[-17].Inthispaperwereportthenucleotidesequenceofth
8、egenomicregionofHCV229Ewhichencodesthemembraneprotein.Thisregionisadjacenttothe3'terminalnucleocapsidpr
