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1、JournalofGeneralVirology(1993),74,1795-1804.PrintedinGreatBritain1795Sequencedeterminationofthenucleocapsidproteingeneoftheporcineepidemicdiarrhoeavirusconfirmsthatthisvirusisacoronavirusrelatedtohumancoronavirus229EandporcinetransmissiblegastroenteritisvirusAnneBridgen,~
2、tMarielaDuarte,2KurtTobler,~HubertLaude2andMathiasAckermann1.1InstitutfiirVirologic,VeteriniirmedizinischeFakultiit,UniversitiitZiirich,Winterthurerstrasse266a,CH-8057Ziirich,Switzerlandand2LN.R.A.,Unit6deVirologicetImmunologicMoldculaires,CRdeJouy-en-Josas,78352Jouy-en-J
3、osasCedex,FranceThenucleotidesequenceof1.7kbpcDNA,comprisingThePEDVNgenewas,however,notablylargerthantheregionnearestthe3'endofthegenomeofthethatofthehuman229Eandporcinetransmissibleporcineepidemicdiarrhoeavirus(PEDV),hasbeengastroenteritisviruses.Thisreflectsthepresenceo
4、faindependentlydeterminedfortwoEuropeanisolatesofputativeinsertionofapproximately135ntlocatedPEDV.AlmostidenticalresultswereobtainedforthetowardsthemiddleoftheNgene.Asecond336ntORF,twoisolates,whichwerederivedfromcasesofPEDVwhichmightencodealeucine-richproteinsimilarto,bu
5、tinfectioninBelgiumandBritainin1977and1987,shorterthan,thebovinecoronavirusinternalproteinrespectively.Thesequencescontaineda1323nucleotidewasfoundwithinthePEDVNgene.SeveralRNAmotifs(nt)openreadingframe(ORF),whichshowedmoderatetypicalofcoronaviruseswerealsoobserved.Thesei
6、dentitytothenucleocapsid(N)geneofothercorona-resultsconfirmtheearlierprovisionalclassificationofviruses.ThegreatestsimilarityatboththenucleicacidPEDVasacoronavirus.andproteinlevelswastothehumancoronavirus229E.Introductionfailedtodetectanyantigeniccross-reactivitybetweenPE
7、DVandothercoronaviruses(Pensaertetal.,1981;Porcineepidemicdiarrhoeavirus(PEDV)causesdiar-Kusanagietal.,1992).However,somecross-reactivityrhoeainpigs,particularlyinneonates.Thediseasehashasbeendetectedwithfelineinfectiousperitonitisvirusbeenrecognizedforapproximately20year
8、s,butthe(FIPV;YalingetaL,1988).Mostrecently,minkseracausativeviruswasfirstdescribedonlyin1978(Pe
