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1、句子与段落翻译：1.TheligatedpasmidmixtureisintroducedintobacterialcellsspeciallytreatedsothattheytakeupDNAinaprocesstermedtransformation.InmostcasesE.coliisthepreferredhostwiththeadvantagesthatcalciumtreatedcellsarehighlytransformable,themolecularbiologyofthisbacteri
2、umiswellunderstoodandavarietyofplasmidandphagevectorsareavailable.Moreover,E.colitranscribesandtranslatesmostGram-positiveandGram-negativegeneswiththeexceptionofsomeactinomycctcgenes.2.Theinsertionsitewithinthevectorislocatedwithinatetracyclineresistancegene.
3、Transformantsthatareampicillinresistantbuttetracyclinesensitivethereforerepresentcellscontainingahybridplasmid(insertioninactivation).3.Fromthisexampleitbecomeclearthat,asinmostdownstreamoperations,theobjectiveoftheprocessingoperationmustbeclearlystated・4.Sev
4、eralgroupshavestudiedacidaccumulationbydifferentstrains,butthebiochemicalmechanismhasnotyetbeencompletelyelucidated・5.Methodsforoxygenanalysisbyparamagneticproceduresandcarbondioxidemeasurementbyinfraredanalysissufferfromthedisadvantageofcalibrationdriftandsl
5、owresponsetime.6.Inthelargersizesofvessel,acontinuousstcam-stcrilizcdsectionofthebearingpreventssuchcontamination,withtheadditionalbenefitoflubricationbysterilecondensate・7・Themostrecent(1984)sellingpricefortheonlycommercialbacterialSCPproductisapproximately$
6、600pertonneforanimalfeedapplicationinWesternEuropeanmarkets.&Utilitiescostsincludingwater,electricityandsteam,laborcosts,maintenanceanddepreciation,taxesandinsurance,workingcapital,interest,anddesiredprofitmustalsobetakenintoaccount.9.Theestimated1976costsof$
7、70millionfor50,000-70,000tonnesperyearcapacitywouldbesubstantiallygreatertoday.1().Themostrecent(1984)sellingpricefortheonlycommercialbacterialSCPproductisapproximately$600pertonneforanimalfeedapplicationinWesternEuropeanmarkets・Theextenttowhichthisproductcan
8、competewithsoybeanmealorfishmealdependsuponcurrentdeliveredpricesforthesecommoditiesinWesternEurope・11.TheannualproductionofmonosodiumL-glutamate,producedexclusivelybyfermentation,exceeds
