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1、凌丹康复方配合DC-CIK治疗白血病临床研究刘清池吴维海李刚荣胡晓东冯新旺梁春耕武大勇李慧慧宋淑花马传宝李建英王荣孝张慧敏牛景月［摘要］目的观察屮药凌丹康复方配合树突状细胞联合细胞因子诱导的杀伤细胞(DC-CIK)治疗口血病的临床效果和安全性。方法病例选择血液学缓解但未达到分子生物学缓解的白血病患者或残留白血病检测阳性患者，其屮急性白血病19例，慢性粒细胞白血病1例。用血细胞分离机采集患者的外周血单个核细胞，分别培养DC和CTK,然后再混合培养获得DC-CTKo将DC-CIK经静脉回输给患者，每15~20天回输一次，共输注
2、4〜6次。同时服用扶正祛邪中药凌丹康复方。观察治疗效果与安全性。结果凌丹康复方配合DGCIK治疗20例缓解期白血病患者,达分子生物学缓解，标志基因转阴4例，其中1例为AML1/ET0,1例CBF/MYH11,1例IgH基因重排，1例bcr/abl；微小残留白血病转为阴性者3例。治疗后3年CR为75%冲位CR期25月(10〜37月)。DC-CIK输注后除5例出现一过性发热，畏寒，无其它严重副作用。结论中药凌丹康复方配合DC・CIK细胞治疗血液学缓解后口血病，具有明显清除微小残留白血病细胞的作用，是血液学缓解后白血病患者合适的
3、选择，静脉输注安全，冇较好的临床应用前景。【关键词】口血病，免疫治疗，树突状细胞，杀伤细胞/细胞因子诱导，中药ClinicalstudyofChineseherbs-lingdankangfufangandDC-CIK(dendriticcellsandcytokine-inducedkiller)combinedtreatmentforleukemiaLIUQing-chi,WUWei~hai,LIGang~rongfHUXiao-dong,FENGXin~wangfLIANGChun-gengWUDa-yong,LIH
4、ui~hui,SONGShu~hua,MAChuan~bao,LIJian-ying,WANGRong-xiao,ZHANGNIUJing-yue1,DepartmentofHematology,Ping'anHospital,2,MolecularBiologyLaboratoryofPing'anHospital,Shijiazhuang050021,ChinaAbstract:Objective:ToevaluatetheeffectivenessandsafetyofChineseherbs-lingdankang
5、fufangandDC・CIK(dendriticcellsandcytokine-inducedkiller)combinedtreatmentofleukemiapatientSeMethods:Selected20patientsachievedhematologicalcompleteremission(HCR)butnotachievedmolecularbiologicalremission(MBR)orminimalresidualLeukemia(MRL)positive.Among20patients19
6、caseswithacuteleukemiaandonecaseswithchronicmyelocyticleukemia..UsedCOBESeparatortoseparatetheperipheralbloodmonocyteofthepatientstoCultureDCandCIKrespective,andthenmixedtoCulture,andthemixedcultureswereintravenousinjectedtothepatientsself.Onceevery15〜20daystointr
7、avenousinject,andthetotalwere4〜6.AllpatientswereadministeredorallywithChineseherbs-lingdankangfufangeveryday.Toobservedtheeffectivenessandsafety.Results:In20patientsreceivedthetreatmentofChineseherbs-lingdankangfufangandDC-CIK,4caseachievedMBR,thatmarkedgenechange
8、negativeincluding2AML1/ETOJCBFB/MYHIland1bcr/abl,and3case(allwereacutenon-lymphocyticleukemia-M2)MRLchangenegative.The3years5CRprobabilityafterChinesehe
