蛋白Marker说明书

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1、PageRulerPrestainedProteinLadder SM0671AprestainedSDS-PAGEMWmarkerwithcontrastingcoloredreferencebandsat10and70kDa.TheThermoScientificPageRulerPrestainedProteinLadderisamixtureoften(10)blue-,orange-andgreen-stainedrecombinantproteins(10to170kDa)foruseassizestandardsinproteinelectrophoresis(SD

2、S-PAGE)andWesternblotting.ThisprestainedproteinMWmarkerisdesignedformonitoringtheprogressofSDS-polyacrylamidegelelectrophoresis,forassessingtransferefficiencyontoPVDF,nylonandnitrocellulosemembranes,andforestimatingtheapproximatesizeofseparatedproteinsthathavebeenmadevisiblewithgelstainsorWes

3、ternblotdetectionreagents.Theladdercontainsoneorangereferencebandat70kDaandonegreenbandat10kDa.Highlights:·Sizerange–10proteinsspanning10to170kDa·Ready-to-use–suppliedinaloadingbufferfordirectloadingongels;noneedtoboil·Sharpbands–color-codedbandsofsimilarintensityforeasyvisualization·Qualityt

4、ested–eachlotevaluatedbySDS-PAGEandWesternblotting·Tworeferencebands–orangeat70kDaandgreenat10kDa·Membrane-compatible–coloredbandstransfertomembranesforWesternblottingIncludes:·Dye-stainedproteinsin62.5mMTris-H3PO4(pH7.5at25°C),1mMEDTA,2%SDS,10mMDTT,1mMNaN3and33%glycerol.Applications:·Monitor

5、ingproteinmigrationduringSDS-polyacrylamidegelelectrophoresis·MonitoringproteintransferontomembranesafterWesternblotting·SizingofproteinsonSDS-polyacrylamidegelsandWesternblotsProductDetails:SDS-PAGEbandprofileoftheThermoScientificPageRulerPrestainedProteinLadder.Imagesarefroma4-20%Tris-glyci

6、negel(SDS-PAGE)andsubsequenttransfertomembrane.SpectraMulticolorBroadRangeProteinLadder Sm1841Aprestained,4-color,SDS-PAGEMWmarkerespeciallyforproteinsbetween10and260kDa.TheThermoScientificSpectraMulticolorBroadRangeProteinLadderisa4-colorproteinstandardcontaining10prestainedrecombinantprokar

7、yoticproteins(10to260kDa)foruseassizestandardsingelelectrophoresisandWesternblotting.ThisprestainedproteinMWmarkerisdesignedformonitoringtheprogressofSDS-polyacrylamidegelelectrophoresis,forassessingtransferefficiencyontoPVDF,nylonandnitrocel