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1、Plant,CellandEnvironment(2011)34,580–591doi:10.1111/j.1365-3040.2010.02264.xUsingtunablediodelaserspectroscopytomeasurecarbonisotopediscriminationandmesophyllconductancetoCO2diffusiondynamicallyatdifferentCO2concentrationspce_2264580..591YOUSHITAZOE1,2,SUSANNEVONCAEMMERE
2、R2,GONZALOM.ESTAVILLO2&JOHNR.EVANS21GraduateSchoolofBiostudies,KyotoUniversity,Sakyo,Kyoto606-8052,Japanand2PlantScienceDivision,ResearchSchoolofBiology,TheAustralianNationalUniversity,Canberra,ACT0200,AustraliaABSTRACTSternberg2000).TheconductancetoCO2diffusionfrominter
3、cellularairspacetothechloroplasthasbeentermedInC3leaves,themesophyllconductancetoCO2diffusion,mesophyllconductance,gm,andhasbeenshowntohaveagm,determinesthedrawdowninCO2concentrationfrommajorinfluenceonthecarbonisotopediscrimination,whichintercellularairspacetothechloropl
4、aststroma.BothgmanddependsontheratioofintercellulartoambientCO2(pi/pa)stomatalconductancelimitphotosyntheticrateandvaryinaswellasCO2assimilationrate(Evansetal.1986,2009;vonresponsetotheenvironment.WeinvestigatedtheresponseCaemmerer&Evans1991;Flexasetal.2007,2008).ofgmtoc
5、hangesinCO2intwoArabidopsisgenotypesItisnoteasytomeasurethemagnitudeofgm.Thetwo(includingamutantwithopenstomata,ost1),tobaccoandmostcommontechniquesrelyoneithercombinedmeasure-wheat.Wecombinedmeasurementsofgasexchangewithmentsofgasexchangeandchlorophyllfluorescenceorcarbo
6、nisotopediscriminationusingtunablediodelasercombinedmeasurementsofgasexchangeandthecarbonabsorptionspectroscopywithaCO2calibrationsystemspe-isotopediscrimination(Ponsetal.2009).ThereisanurgentciallydesignedforarangeofCO2andO2concentrations.needtoassesstowhatextentgmislin
7、kedtoleafanatomicalCO2wasinitiallyincreasedfrom200to1000ppmandthenpropertiessuchaschloroplastsurfaceareaappressedtodecreasedstepwiseto200ppmandincreasedstepwisebackintercellularairspaceandcellwallthickness(Evansetal.to1000ppm,orthesequencewasreversed.In2%O2astep2009)andt
8、owhatextentgmvarieswithshort-termenviron-increasefrom200to1000ppmsignificantlydecreasedgmmentalperturbat
