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1、Interferenceterahertzlabel-freeimagingforproteindetectiononamembrane1,*2,1YuichiOgawa,,Shin’ichiroHayashi,324,2,1MasatoOikawa,,ChikoOtani,andKodoKawase,1GraduateSchoolofAgriculturalScience,TohokuUniversity,Tsutsumidori-Amamiya-machi,Aoba-ku,Sendai981-8555,Japan2RIKENSENDAI,519-1399Arama
2、kiaoba,Aoba,Sendai980-0845,Japan3GraduateSchoolofLifeSciences,TohokuUniversity,Tsutsumidori-Amamiya-machi,Aoba-ku,Sendai981-8555,Japan4EcoTopiaScienceInstitute,NagoyaUniversity,Furo-cho,Nagoya464-8603,Japan*Correspondingauthor:yogawa@bios.tohoku.ac.jpAbstract:Wedemonstrateahighlysensiti
3、veimagingmethodcombinedaterahertztime-domainspectroscopyandaninterferenceeffectforlabel-freeproteindetectiononapolyvinylidenedifluoridemembrane.Themethodisbasedonterahertztime-domainspectroscopyandusesaninterferenceeffect.Biotinislinkedtothemembraneusingpolyethyleneglycolorpolyethyleneg
4、lycolmethylethertopreventitfrombeingwashedoff.Bindingofthebiotinwithstreptavidinisthenobservedbymeasuringtheterahertzsignalchangeduetothevariationofthemembranerefractiveindex.Wedemonstratethedetectionofthebindingstreptavidinproteiningradually-2decreasingconcentrations,downto27ngmm,using
5、theimagerecordedat1.5THz.©2008OpticalSocietyofAmericaOCIScodes:(170.6795)Terahertzimaging;(170.3880)Medicalandbiologicalimaging;(110.6795)Terahertzimaging.Referencesandlinks1.M.Schena,D.Shalon,R.W.Davis,andP.O.Brown,“QuantitativeMonitoringofGeneExpressionPatternswithaComplementaryDNAMic
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7、ll-MoleculeInteractions,”Angew.Chem.Int.Ed.42,5584-5587(2003).4.S.Fukui,T.Feizi,C.Galustian,A.M.Lawson,andW.Chai,“Oligosaccharidemicroarraysforhigh-throughputdetectionandspecificityassignmentsofcarbohydrate-proteininteractions,”NatureBiotechnology20,1011-1017(2002).5.J.M.McDonn
