protocol_ Primer Design

《protocol_ Primer Design》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。

1、homecoursematerialsresearchmembersmultimedialiterature第1页共6页LastUpdatedApril20,201111:40AMAuthoredBy:KatieLuethckeDesigningprimerscomesinhandy,whetheryouaretryingtoamplifyageneforqualitativeexpressionanalysisintreatment/non-treatmentcultures,orjustsi

2、mplytryingcheckforageneyou’vedeleted.Thefirststeptoasuccessfulgeneamplificationisdesigningyourprimers,so,let’sgetstarted!Step1:LookupyourgeneintheSaccharomycescerevisaegenomebrowser.SimplytypeinthenameofthegeneyouwishtoamplifyduringyourPCRorRT-PCR,an

3、dhitgo!Forexample,IamlookingtoamplifyMEC3.Youshouldgetsomethinglikethis:第2页共6页Goaheadandscrolldowntothebottomofthepage,untilyougettoasubheadinginthelefthandcolumntitled“SequenceInformation.”Thisistheareawhereyouwillfindthelocusofyourgene第3页共6页第4页共6页第

4、5页共6页第6页共6页