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1、OriginalArticleIdentificationofDifferentiallyExpressedGenesinBreastTumorsFromAfricanAmericanComparedWithCaucasianWomen12133LoriA.Field,PhD;BradLove,PhD;BrendaDeyarmin,HT;JeffreyA.Hooke,MD;CraigD.Shriver,MD;4andRachelE.Ellsworth,PhDBACKGROUND:BreasttumorsfromAf
2、ricanAmericanwomenhavelessfavorablepathologicalcharacteristicsandhighermortalityratesthanthoseofCaucasianwomen.Althoughsocioeconomicstatusmayinfluenceprognosis,bio-logicalfactorsarealsolikelytocontributetotumorbehavior.METHODS:Patientswithinvasivebreastcancerw
3、erematchedbyage,grade,andestrogenreceptorstatus;patientswithbenigndiseasewerematchedbyageanddiag-nosistype.RNAfromlasermicrodissectedtumorsandwhole-sectionednonmalignantbreasttissueswashybridizedtoHGU133A2.0microarrays.DatawereanalyzedusingPartekGenomicsSuiteu
4、singacutoffofP<.001,>1.5-foldchange,andresultswerevalidatedbyquantitativereal-timepolymerasechainreaction.RESULTS:Clinicopathologicalfactorsdidnotdiffersignificantlybetweengroupsforageatdiagnosis,tumorsizeorstage,lymphnodeorhumanepidermalgrowthreceptor2status,
5、intrinsicsubtype,ormortality.Two-wayanalysisofthetumorspecimensrevealed25probesrepresenting23genesdifferentiallyexpressedbetweenpopulations;hierarchicalclusteringclassified24of26AfricanAmericanwomenand25of26Caucasianwomencorrectly.Inthenonmalignantspecimens,15
6、probesrep-resenting13genesweredifferentiallyexpressed,including5genesthatalsodifferedinthetumorspecimens;thesegeneswereabletocorrectlyclassifynonmalignantbreastspecimensfrom20of22ofAfricanAmericanwomenandalloftheCaucasianwomen.CONCLUSIONS:Despitematchingoftumo
7、rsbypathologicalcharacteristics,molecularprofilesdifferedbetweenAfricanAmericanwomenandCaucasianwomeninbothinvasivetumorsandbenignbreasttissues.Thesedifferentiallyexpressedgenes,includingCRYBB2,PSPHL,andSOS1,areinvolvedincellulargrowthanddifferentiation,invasi
8、on,metastasis,andimmuneresponseandthusmaycontributetothepooroutcomeinAfricanAmericanwomen.Cancer2012;118:1334-44.VC2011AmericanCancerSociety.KEYWORDS:breastcancer,AfricanAmerican,d