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1、·846·HeraldofMedicineVol126No18August2007灯盏生脉胶囊抗大鼠局灶性脑缺血2再灌注损伤作用研究李浩,贾建平(首都医科大学宣武医院神经内科,北京100053)[摘要]目的研究灯盏生脉胶囊抗大鼠局灶性脑缺血2再灌注损伤的作用。方法将58只雄性SD大鼠随机分为4组,即假手术组10只,0.9%氯化钠溶液组和灯盏生脉胶囊高、低剂量组各16只。假手术组行假手术,其他3组制作大脑中动脉缺血2再灌注模型。假手术组和0.9%氯化钠溶液组于术前72,48,24,0.5h及术后22h灌胃给予0.9%氯21化钠溶液,
2、灯盏生脉胶囊低、高剂量组在相同时点分别按0.36和0.72g·kg剂量灌胃给予灯盏生脉胶囊内容物悬浊液4mL;各组大鼠均在最后一次给药后2h处死,于脑缺血2h和再灌注2和22h分别进行神经行为学评分,再灌注22h后测定大鼠血清乳酸脱氢酶活性及脑梗死体积百分比,观察脑组织病理学改变。结果再灌注2h后,灯盏生脉胶囊高剂量组神经行为学评分[(1.75±0.68)分]较0.9%氯化钠溶液组[(2.31±0.79)分]明显降低(P<0.05);再灌注22h后,灯盏生脉胶囊高剂量组神经行为学评分[(1.63±0.89)分]明显低于0.9%氯化
3、钠溶液组[(2.38±1.09)分](P<210.05)。再灌注22h后,灯盏生脉胶囊高剂量组血清乳酸脱氢酶活性[(1331.89±366.85)U·L]明显低于0.9%氯化-1钠溶液组[(1799.56±325.92)U·L](P<0.05),灯盏生脉胶囊高剂量组脑梗死体积百分比[(9.73±5.66)%]亦明显低于0.9%氯化钠溶液组[(18.31±6.96)%](P<0.05)。灯盏生脉胶囊低、高剂量组鼠脑组织缺血周围区细胞周围水肿、神经元变性和间质破坏较其他各组轻。结论灯盏生脉胶囊有一定的抗大鼠局灶性脑缺血2再灌注损伤作用
4、,作用机制可能在于抑制缺血半暗带恶化。高剂量作用更明显。[关键词]灯盏生脉胶囊;脑缺血2再灌注损伤;[中图分类号]R286[文献标识码]A[文章编号]100420781(2007)0820846205EffectofDengzhanShengmaiCapsulesonFocalCerebralIschemia-reperfusionInjuryinRatsLIHao,JIAJian2ping(DepartmentofNeurology,XuanwuHospitalofCapitalUniversityofMedicalScienc
5、es,Beijing100053,China)ABSTRACTObjectiveTostudytheeffectofdengzhanshengmaicapsulesonthefocal2reperfusioninjuryinrats.Methods58maleSprague2Dawleyratswererandomlydividedinto4groups,shamoperationgroup(10),physiologicalsalinegroup(16),groupoflow2dosage(16)andgroupofhigh2d
6、osageofdengzhanshengmai(16).Shamoperationwasperformedinshamoperationgroup,andmiddlecerebralarteryischemia2reperfusionmodelwasestablishedintheother3groups.4mLof210.9%sodiumchloride,0.36and0.72g·kgofsuspensionofdengzhanshengmaicapsulesweregivenbyintragavageat72,48,24,0.
7、5hbeforeoperationand22hafteroperationinshamoperation,physiologicalsaline,lowdoseandhighdosegroup,respectively.Ratsweresacrificed2hafterthelastdrugadministration,neurologicalbehaviorevaluationwasdoneforratsatdifferenttime,2haftercerebralischemiaand2h,22hafterreperfusio
8、n.Lactatedehydrogenase(LDH)activityinserum,thepercentageofcerebralinfarctsizeandneuropathologichistopathologicalchangeswereo
