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1、上海交通大学学报(医学版)Vol.26No.7Jul.2006JournalofShanghaiJiaotongUniversity(MedicalScience)·725·【文章编号】0258-5898(2006)07-0725-05·基础研究·丘脑底核高频电刺激对大鼠黑质-纹状体多巴胺代谢的影响1121赵永波,王乔树,孙伯民,郭春妮12(上海交通大学第一人民医院神经内科,上海200080;上海交通大学医学院瑞金医院神经外科)【摘要】目的研究丘脑底核(STN)高频电刺激(HFS)对大鼠黑质-纹状体系统的影响。方法给予正常大鼠一侧STN2HFS,应用微透
2、析观察其对纹状体多巴胺(DA)及其代谢产物的影响,应用逆转录-聚合酶链反应(RT2PCR)和Westernblot观察其对黑质、纹状体酪氨酸羟化酶(TH)的影响。结果刺激侧纹状体DA代谢产物明显增多(P<0.05),DA水平无变化;RT2PCR检测发现刺激侧黑质、纹状体THmRNA水平升高;Westernblot检测发现刺激侧黑质TH表达无变化,而纹状体TH表达明显升高(P<0.01)。结论STN2HFS可能通过影响黑质-纹状体DA代谢及其限速酶的水平发挥作用。【关键词】帕金森病;高频电刺激;酪氨酸羟化酶;微透析;丘脑底核【中图分类号】R742.5【文献
3、标识码】AEffectsofSTN2HFSonSubstantiaNigraandStriatumDopaminergicMetabolisminRats1121ZHAOYong2bo,WANGQiao2shu,SUNBo2min,GUOChun2ni1(DepartmentofNeurology,TheFirstPeople’sHospital,ShanghaiJiaotongUniversity,Shanghai200080,China;2DepartmentofNeurosurgery,RuijinHospital,SchoolofMedicine
4、,ShanghaiJiaotongUniversity)Abstract:ObjectiveToidentifythemechanismsofthesubthalamicnuclei(STN)highfrequencystimulation(HFS)forParkinson’sdisease(PD).MethodsWeinvestigatedtheeffectsofSTN2HFSonstriataldopaminergicmetabolismusinginvivomicrodialysisinthefreelymovingrats.Furthermore
5、,reversetranscription2polymerasechainreactionandWesternblotofstriatalandnigraltyrosinehydroxylase(TH)wereperformed.ResultsSTN2HFSin2creasedstriataldopaminergicmetabolismandenhancedstriatalTHproteinandgeneexpressionwithoutaffectingTHproteinexpressioninsubstantianigra.ConclusionThe
6、applicationofSTN2HFScouldcompensateforthedopa2minedeficitbyinducingincreasedstriataldopaminemetabolismandmodificatingproteinorgeneexpressionoftherate2limitingenzyme.Keywords:Parkinsonpsdisease;highfrequencystimulation;tyrosinehydroxylase;microdialysis;subthalamicnucleus丘脑底核(subth
7、alamicnucleus,STN)高频电刺激低基底节传出结构黑质网状部(substantianigrareti2(highfrequencystimulation,HFS)已经被广泛应用于culate,SNr)和苍白球内侧部(globuspallidusinter2帕金森病(Parkinson’sdisease,PD)的治疗,其对PDnal,GPi)的兴奋性,而低频电刺激(LFS)作用相[3]的主要症状包括运动迟缓、震颤、僵直均有改善作反。但STN2HFS对于多巴胺(dopamine,DA)能递[1][2,4]用,尤其是常规药物治疗疗效欠佳的患者。但质
8、的影响知之甚少,并得出相反的研究结果。临[5]HFS的精确机制尚未阐明,一般认为
