生物染色剂试纸法快速测定水样中的Pb_[1]

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1、农业环境科学学报2006,25(4):1006-1009JournalofAgro-EnvironmentScience生物染色剂试纸法快速测定水样中的Pb(Ⅱ)赵秀杰1,2,徐应明1,费学宁2,郭玉香1,3,戴晓华1(1.农业部环境保护科研监测所,农业环境与农产品安全重点开放实验室,天津300191;2.天津城市建设学院市政与环境工程系,天津300384;3.天津理工大学生物与化学工程学院,天津300191)摘要:为了快速测定水样中的微量Pb(Ⅱ),将生物染色剂孔雀绿(BG)浸渍在定量滤纸上制备成铅检测试纸

2、并研究了试纸与Pb(Ⅱ)的反应条件。结果表明,在水样中依次加入抗坏血酸、KI和HNO,调解其浓度分别为0.010、0.15和0.40mol·L-1,Pb(Ⅱ)与试纸3上的生物染色剂孔雀绿反应生成绿蓝色的三元离子缔合物BG2[PbI4],该缔合物的颜色与水样中Pb(Ⅱ)浓度成正比,对Pb(Ⅱ)的检出限为0.50mg·L-1。水样中的一般干扰离子可用0.5%的半胱氨酸掩蔽。该试纸对Pb(Ⅱ)具有良好的选择性,测定重复性好、操作过程简便,完全可以满足水样中Pb(Ⅱ)的现场快速检测需要。关键词:生物染色剂;孔雀绿;试

3、纸;铅;快速测定;水样中图分类号:X853文献标识码:A文章编号:1672-2043(2006)04-1006-04Rapid-determinationofPb(Ⅱ)inWaterSampleswithBiologicalDyeTestPaperZHAOXiu-jie1,2,XUYing-ming1,FEIXue-ning2,GUOYu-xiang1,3,DAIXiao-hua1(1.KeyLaboratoryofAgro-EnvironmentandAgro-productsafety,Agro-Envi

4、ronmentalProtectionInstitute,MOA,Tianjin300191,China;2.TianjinInstituteofUrbanConstruction,Tianjin300384,China;3.TianjinUniversityofTechnology,SchoolofBiotechnologyandChemicalEngineering,Tianjin300191,China)Abstract:Atestpapermethodforrapiddeterminationofle

5、adinwatersampleswasproposed.Thepreparationofthetestpaperandit'sap-plicationconditionswerestudied.Theprocedureofpreparingthetestpaperwasasfollows:firstly,fivepiecesofmid-velocityquantitativefil-terpapersweredippedinto100milliliterworkingfluidofbrilliantblue,

6、akindofbiologicaldye,atthepercentageconcentrationof0.1%for30min,thendriedwithoutbrightlightandatlastbeingcutintosmallpiecesof8mm×6mm,calledleadtestpaper.Beforethetestpaperbe-ingappliedinleaddetermination,watersampleshadtobetreatedbyascorbicacid,potassiumiod

7、ideandnitricacidinadvance,thebestconcentrationsofeachwere0.010mol-1-1-1·L,0.15mol·Land0.40mol·Lrespectively.Aftersomeofthetreatedwatersamplesweretrans-mittedonthetestpaper,lead,potassiumiodideandbrilliantbluereactedonthepapertogenerateagreen-blueassociateco

8、mplexandthedepthofthecolorwaspositivelycorrelatedwithleadconcentration.Thedetectionlimitofthemethodwas0.50mg-1.Theinterferingions·Lcouldbecoveredby0.5%cysteine.Thetestpapermethodwassimple,rapid,selecti