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1、论著内生真菌对福建金线莲栽培及酶活性的影响*唐明娟,孟志霞,郭顺星,陈晓梅,肖培根(中国医学科学院中国协和医科大学药用植物研究所,北京100094)摘要:目的研究在野外盆栽条件下内生真菌AR18对福建金线莲(Anoectochilusroxburghii)生长发育的影响。方法福建金线莲组培苗接菌移栽于草炭土或蛭石基质中,1年后收获,观察苗的生长势,统计成活率,称鲜重和干重,同时测定1,3葡聚糖酶、几丁质酶、多酚氧化酶和苯丙氨酸解氨酶活性。结果在草炭土和蛭石基质上,接菌的福建金线莲苗成活率均达100%;真菌对福建金线莲鲜重的增加有极显著促
2、进作用(P<001),分别为对照组的177和183倍;真菌对福建金线莲干重的增加有显著促进作用(P<005),分别为对照组的113和128倍。接菌后4种酶活性均高于对照组。结论在福建金线莲的家栽过程中可利用内生真菌提高成活率和产量。关键词:内生真菌;福建金线莲;移栽;1,3葡聚糖酶;几丁质酶;多酚氧化酶;苯丙氨酸解氨酶中图分类号:S567文献标识码:A文章编号:1001-2494(2008)12-0890-04EffectsofEndophyticFungiontheCultureandFourEnzymeActivities
3、ofAnoectochilusroxburghii*TANGMingjuan,MENGZhixia,GUOShunxing,CHENXiaome,iXIAOPengen(InstituteofMedicinalPlantsDevelopment,ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,Beijing100094,China)ABSTRACT:OBJECTIVETostudytheeffectofendophyticfungiAR18(Epulorhizasp)o
4、nthegrowthofAnoectochilusroxburghiiMETHODSAroxburghiiwasincubatedwithendophyticfungiAR18,harvestedafterbeingculturedforageinpeatorvermiculitemediumSeedlingsgrowthvigorwereobserved,statisticsweretaken,freshweightanddryweightweregained,andenzymeactivitiesof1,3glucanase,chit
5、inase,polyphenoloxidase(PPO)andphenylalanineammonialyase(PAL)weredeterminedRESULTSInpeatandvermiculitemedium,thesurvivalratesofAroxburghiiincubatedwithendophyticfungiwereboth100%ThefungiextremelysignificantlyincreasedthefreshweightofAroxburghiiseedlings(P<001),whichwas177t
6、imesand183timesasthatofthenoninoculatedones,respectivelyThefungisignificantlyincreasedthedryweightofseedlings(P<005),whichwas113timesand128timesascontro,lrespectivelyThefourenzymeactivitieswereenhancedintheseedlingsincubatedwithendophyticfung,icomparedwiththecontrolCONCLUSI
7、ONSurvivalratesandyieldofAroxburghiicanbeincreasedbythetransplantationcultivationofendophyticfungiKEYWORDS:endophyticfung;iAnoectochilusroxburghii;transplanting;1,3glucanase;chitinase;polyphenoloxidase;phenylalanineammonialyase福建金线莲(Anoectochilusroxburghii)是兰科开世纪90年代开始了对金
8、线莲的试管苗快繁和人工唇兰属多年生草本植物,在我国主
