青稞遗传多样性及其农艺性状与SSR标记的关联分析

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1、作物学报ACTAAGRONOMICASINICA2016,42(2):180189http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00180青稞遗传多样性及其农艺性状与SSR标记的关联分析1,21,21,21,21,21,2孟亚雄孟林祎汪军成司二静张海娟任盼荣1,21,31,21,2*马小乐李葆春杨轲王化俊12甘肃省干旱生境作物学重点实验室/甘肃省作物遗传改良与种质

2、创新重点实验室,甘肃兰州730070;甘肃农业大学农学院,甘肃3兰州730070;甘肃农业大学生命科学技术院,甘肃兰州730070摘要:利用92个SSR标记对108份青稞亲本材料进行多态性扫描,分析其遗传多样性,旨在寻找与农艺性状相关联的分子标记,为青稞杂交组合的配制及分子标记辅助育种提供依据。挑选48个多态性标记进行群体遗传结构分析,在此基础上采用Tassel2.1GLM(generallinearmodel)和MLM(mixedlinearmodel)方法进行标记与农艺性状的关联分析。共检测出156个等位变异,每个位点2~6个等位变

3、异。供试群体的Shannon指数为0.6727~1.1368,材料间遗传相似系数为0.2250~1.0000,平均0.7585。通过群体遗传结构分析将供试材料划分成4个亚群。以GLM分析,发现12个与株高、穗长、穗粒数和分蘖数相关联的标记,对表型变异的解释率分别为11.5%~17.6%、19.4%~45.4%、15.4%~22.1%和29.2%;以MLM分析,发现8个与株高、分蘖数和小穗数相关的标记,各标记对表型变异的解释率分别为31.7%~49.8%、28.1%~37.2%、22.7%~32.7%。关联标记分布在基因组全部6个连锁群上

4、。关键词:青稞;SSR;遗传多样性;群体遗传结构;关联分析GeneticDiversityandAssociationAnalysisofAgronomicCharacteristicswithSSRMarkersinHullessBarley1,21,21,21,21,2MENGYa-Xiong,MENGYi-Lin,WANGJun-Cheng,SIEr-Jing,ZHANGHai-Juan,REN1,21,21,31,21,2,*Pan-Rong,MAXiao-Le,LIBao-Chun,YANGKe,andWANGHua-Jun1

5、GansuProvincialKeyLaboratoryofAridlandCropScience/GansuKeyLaboratoryofCropImprovement&GermplasmEnhancement,Lanzhou23730070,China;CollegeofAgronomy,GansuAgriculturalUniversity,Lanzhou730070,China;CollegeofLifeSciencesandTechnology,GansuAgriculturalUniversity,Lanzhou730070

6、,ChinaAbstract:Theobjectivesofthisstudyweretofindmolecularmarkersassociatedwithyield-relatedtraitsandguideparentalcombinationinmolecularmarker-assistedbreedingandhybridbreedingofhullessbarley(HordeumvulgareL.var.nudumHK.f.).Anaturalhullessbarleypopulationcomposedof108par

7、entalvarieties/lineswasscreenedwith92SSRmarkers,inwhich48markerswerepolymorphic.PopulationstructurewasanalyzedbasedonthepolymorphicSSRdataandassociationbetweenmarkersandfiveagronomictraitswereperformedinTASSELGLM(generallinearmodel)andMLM(mixedlinearmodel)programs.Atotal

8、of156allelesweredetectedinthe108varieties/lineswith2–6allelesperlocus.TheShannon’sindexofthepopulationr