不饱和脂肪酸海藻糖单酯酶法合成及水相氧化研究

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1、江南大学博士学位论文不饱和脂肪酸海藻糖单酯酶法合成及水相氧化研究姓名：孙月娥申请学位级别：博士专业：食品科学指导教师：夏文水20090601江南入学flI}Ij学化论义ifffg[e：力、CMC等界嘶性质的影响。结果表明，酰基链上碳原-了数增加使CMC值减小，酰基链上双键数增加使CMC值增人，温度对／fi饱和脂肪酸海藻糖单酯CMC的影响很小，随着温度升高，CMC值略有下降。研究了油酸海藻糖单酯、业油酸海藻糖单脂、亚麻酸海藻糖单酯、EPA海藻糖单酯和DHA海藻糖单酯在水溶液巾的氧化行为。考察了酰琏链上双键数量、脂肪酸链长以及不

2、『刊浓度对不饱和脂肪酸海藻糖单酯在水溶液中氧化jJ：为的影响、并分析了氧化与不饱和脂肪酸海藻糖单酯结构的关系。结果表明，当不饱和脂肪酸海藻糖译酯存水溶液中以单体状态存在时，其稳定性递减顺序为：油酸海藻糖单酯、亚油酸海藻糖r毪酯、亚麻酸海藻糖币．酯、EPA海藻糖译酯和DHA海藻糖币．哺；半不饱和脂肪酸海藻糖单酯在水溶液中以胶束状态1竽住时，其稳定性递增顺序为：业麻酸海藻糖单酯、业油酸海藻糖单酯、油睃海藻糖单酯、EPA海藻糖单酯和DHA海藻糖单酯。关键词：不饱和脂肪酸海藻糖竹酯；．{卜水相合成；脂肪酶；表面性质；水溶液；胶束；氧

3、化行为AbstrabtAbstractCarbohydratefattyacidestersisakindofnonionsurfactant，whichismadefromrenewablefeedstockanddoesharmlesstotheenvironment．Duetotheirfunctional．nontoxicandnonskin-irritantproperties，tlleyhavebeenwidelyusedinfood，pharmaceutical，cosmeticanddetergentindu

4、stries．Becausetheobviouslydefectsofchemicalsynthesis，weadoptbio—catalyticprocesstosynthesizethecarbohydratefattyacidesters．Mostcarbohydratefattyacidesterssaledonmarketarefromsaturatedfattyacidandsugar．However，unsaturatedfatttyacidhavemanybiologicalactivitiesandphys

5、iologicalfunctionsandothermerits，sotheresearchoncarbohydrateunsaturatedfattyacidestersattracttheinterestofmanyresearchers．LipidswithhighPI『FAscontentaresusceptibletooxidationanditisreportedthattheoxidationofailunsaturatedsubstrateinaqueoussolutionisdifferentfromtha

6、tinbulkoil．Ⅵ佬studiedonthesynthesisandpropertiesofcarbohydrateunsaturatedfattyacidestersfirstly，theninvestigatedtheiroxidationinaqueoussolution．Themainresultsareasfollows：Fivecarbohydratefattyacidestersweresynthesizedfromtrehaloseandunsaturatedfattyacidsortheirethyl

7、estersintert．butanolusingmicrobiallipaseasabiocatalystinabatchstirredtankreactor．Wesystematicallyinvestigatedtheeffectofmanyfactorssuchasreactiontime，molecularsieves，solvent，temperature，lipase，substrateconcentrationonthesynthesisofcarbohydratefattyacidesters．Theopt

8、imalconditionfornonaqueoussynthesisofmonoacyltrehalosewasasfollows：trehaloseconcentration13．2mm01／1．1inoleicacidconcentration41．7mmol／l，molecular