下丘脑室旁核orexin-a对大鼠体重和自由活动的影响及机制

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1、分类号：Q617密级：无UDC：570学校代码：11065硕士学位论文论文题目下丘脑室旁核orexin-A对大鼠体重和自由活动的影响及机制杨丽媛指导教师徐珞教授学科专业名称病理生理学论文答辩日期2015年11月28日摘要目的：探讨室旁核（PVN）微量注射orexin-A对大鼠体重的影响及机制。方法：采用PVN埋管、PVN微量注射，EchoMRI900体成分分析仪分析身体成分等方法，观察室旁核微量注射orexin-A对大鼠体重、身体成分和自由活动的影响。结果：与注射人工脑脊液对照组相比，下丘脑PVN微量注射orexin-A，大鼠体重明显减轻（P<0.05），但大鼠摄食量无明显差异(P﹥0.05

2、)。实验结束后6天，PVN微量注射orexin-A组大鼠体重仍显著低于注射前（P<0.05），而人工脑脊液对照组大鼠体重比注射前显著增加（P<0.05）；与注射人工脑脊液对照组大鼠比较，PVN微量注射Orexin-A，大鼠肌肉组织、脂肪组织和非脂肪组织均显著降低（P<0.05），但注射orexin-A的大鼠降低更明显。与注射人工脑脊液对照组相比，PVN微量注射orexin-A，大鼠的摄食转化率（摄食量/体重）显著降低（P<0.05）。下丘脑PVN微量注射orexin-A，大鼠水平运动量显著增加（P<0.05）。结论：室旁核orexin-A可通过增加大鼠活动量而产生负能量平衡，体重减轻。硕士研

3、究生：杨丽媛（病理生理学）指导教师：徐珞教授关键词：Orexin-A；PVN；非运动性活动产热；负能量平衡；体重ABSTRACTObjective:Toinvestigatetheeffectoforexin-Amicroinjectedintotheparaventricularnucleusofthehypothalamus(PVN)onbodyweightinrats.Methods:ChronicguidecannulaewereimplantedintoPVNofrats.Orexin-Awasmicroinjectedintothebrainsite.Theeffectofore

4、xin-Aonbodyweightinratswasdetected.Results:Thelossofbodyweightinorexin-A-treatedratswasmoresignificantlythantheirvehicle-injectedcounterparts（P<0.05）,buttheyhadnodifferenceinfoodintake(P﹥0.05).Theratsweremoresignificantlyactiveafterintra-PVNorexinmicroinjectioncomparedtovehicle（P<0.05）.Theseresults

5、supporttheconceptthatorexinergicagentshavethepotentialtoproducenegativeenergybalancethroughincreasingphysicalactivity.Conclusion:Orexin-Amicroinjectedintotheparaventricularnucleusofthehypothalamus(PVN)caninduceweightlossinratsthroughproducingnegativeenergybalanceviatheincreaseofphysicalactivity.Pos

6、tgraduatestudent:Li-YuanYang(Pathophysiology)Directedby:Prof.LuoXuKeywords：Orexin-A;PVN;Non-exerciseactivitythermogenesis;Negativeenergybalance;Bodyweight目录引言······································································································1第1章材料与方法······························

7、····················································31.1主要实验材料·····················································································31.2主要实验方法·················································