资源描述：
《微生物生态学》由会员上传分享，免费在线阅读，更多相关内容在教育资源-天天文库。
1、SoilBiology&Biochemistry42(2010)1148e1156ContentslistsavailableatScienceDirectSoilBiology&Biochemistryjournalhomepage:www.elsevier.com/locate/soilbioMetaproteomiccharacterizationofasoilmicrobialcommunityfollowingcarbonamendmentMarkA.Williams*,ErinB.Taylor,Him
2、ayaP.MulaDepartmentofPlantandSoilSciences,MississippiStateUniversity,470DormanHall,MississippiState,MS39762,USAarticleinfoabstractArticlehistory:Metaproteomicshasthepotentialtotransformthestudyofmicrobialcommunityfunctioninginsoil.ToReceived18December2009test
3、thispotential,experimentswereconductedtocharacterizethemicrobialcommunityproteomeinReceivedinrevisedform(1.)toluene-amendedsoiland(2.)toluene-amendedsoilmicrobialinoculatedcultures.Aglucose-22March2010amendedsoilwasalsousedasacontrasttotheeffectsoftoluene.Ato
4、talof47proteinswereidentifiedatAccepted23March2010a95%confidenceinterval.Thetoluene-amendedcommunitiessharedmanyproteinsandshowedahighAvailableonline9April2010degreeofsimilaritytopreviousstudiesoftolueneimpactedbacteria.Forexample,themicrobialcommunitiesintheto
5、luene-amendedsoilandculturesbutnottheglucoseimpactedmicrobialKeywords:communities,showedthepresenceofglutaminesynthetase(Gln),ABCtransporters(e.g.Glt1),extra-SoilmetaproteomeProteincellularsolute-bindingproteins,andoutermembraneproteins(Omp).Theseproteinsmayb
6、eupregu-SDS-PAGElatedforthepurposeofremovingtoluenefrombacterialcells.Toluene-amendedculturesalsoshowedSoilmicrobialcommunitythepresenceofargininedeiminase(ArcA),andcold-shockprotein(Csp),whilemicrobialproteinfrom16SrRNAgenestoluene-amendedsoilshowedthepresen
7、ceofthestressproteinssuperoxidedismutase(SodB)andDensitygradientcentrifugationchaperonin(GroEL).Fattyacidmethylesteranalysisindicatedthattoluene-exposedmicroorganismsincreasedtherigidityoftheirmembranesthrougha3to12increaseintheratioofsaturatedtomonounsatura
8、tedfattyacids.Moreover,the16SrRNAgeneanalysisofbacterialcommunitiesinthetolueneamendedsoilshowedahighdegreeofdominance,wherebyw80%oftheOTUsweredescribedbyrepresentativesmostcloselyrelated
