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1、wound,afterbeingitsnaturalfilm,bindupandfixed.GroupBafterinjury,OnlysprayingEGFonthewound,untilsolutionnotoutflow,bindupandfixed.GroupCafterinjury，onlysprayingchitosanfilmformeronthewound,afterbeingitsnaturalfilm,bindupandfixed.Every24hoursreplacethedrugandouterdressing,ma
2、croscopicobservationthreegroupsinmorphologicalchangesandhealingofwounds.Selectingobservationpointof3d,7d,10d,14d,eachobservationpointrandomlyextractsixrabbits,injecttheairintotherabbitstodeath,useweighingpaperrecordthewoundarea,andcalculatetherateofwoundhealing.underthestr
3、ictasepticoperation,cutoutthewoundescharandundereschartissuemass1.0gram,Processingafterbacterialculture,tosumupwoundtissuecoloniesofbacteriapergram.Thentakethe100mgrabbitbackwoundtissue,determinationofthewoundtissuehydroxyproline(Hyp)content.Therestofthespecimentissue,onep
4、artsofthetissuesliceandmakeHEdyeing,observingpathologicalindicators,otherpartsoftissuemakeki67specificantibodydyeing,observetheimmunohistochemicalindex.Results:Observingthewoundhealinginmacroscopicview,woundgroupAcomparedwithBandCgroups,aroundthewoundinflammationreactionli
5、ghter,escharsoftandlooser,Woundareareducefaster.Comparisonofthewoundhealingrate,GroupAissignificantlyhigherthanBandCgroups,groupsBandgroupCarenotobviousdifferencebetweentwogroups.Histopathologicalspecimensofthewound4observeUnderthemicroscope,woundgroupAcomparedwithBandCgro
6、ups,necrotictissueattachedlooseofthewound,inflammatorycellchemotaxisgatheredspeed,endothelialcellshyperplasiaobviously,collagenfibershyperplasiaactive,newepitheliumarrangementneatly.ColonyFormingUnits/gram(CFU/g)positivedetectionrateingroupBwashigherthangroupA,Positiverate
7、ingroupBwashigherthangroupC,AandBgroupsnosignificantdifference.Detectionofthewoundtissuehydroxyproline(Hyp)content,AgroupofHypcontentisgrowingfasterthangroupB,CgroupofHypcontentisgrowingfasterthangroupB,AandCgroupsnosignificantdifference.Immunohistochemicalanalysisforki67,
8、woundgroupAcomparedwithBandCgroups,largenumberofpositivecellsgatheredveryclosely,thenucle
