食品中甾体激素残留免疫分析技术与方法分析

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1、江南人学博I：学位论文pHofsampletestingshouldbeadjustedinthe7．0～8．0region，organicsolventcontentinsamplesshouldbecontrolledat30％orless，theionicstrengthofsamplesshouldbecontrolledinO．1mol／Lbelow．Thedetectionlimitwas5ng／mLfordetectionof19-NTstandardsolution．Throughanacceleratedstabil

2、itytest．wecanpredictthattheteststripCanbestabilizedforatleast5monthsatroomtemperature．1mmol19-NTasthetemplatemolecule．4mmolmethacrylicacidasfunctionalmonomer,lOmmolTRIMascrosslinker,werepreparedfor24hat60℃byprecipitationpolymerizationformolecularlyimprintedpolymermicros

3、pheres(MIPs)，whichhasrelativelyuniformparticlesize．Thespecificsurfaceareawas40．2421m2／g，theporesizewasabout20．3rim．Themaximumapparentbindingcapacityof94．882pmol／gto19·NT，theidentifyfactorpvalueswas27．26forthetemplatemoleculewhichshowedthestrongestbindingcapacityandspeci

4、ficityofrecognition．ElutionoftheMIP-SPEconditionswereoptimized，10％methanolwasusedaslotion，methanol／aceticacid(9：1，V／V)selectedaseluent，whenuse4mLeluate，almostalladsorbed19-NTmoleculecanbeelutedoff．Atthe1thuseofMIP·SPE．theaveragerecoveryratecanstillreach85％ormore，theenti

5、reprocessofCVvaluesarelessthan5％．ThelinearequationofMIPcolumnfor19-NTWasY=0．4188X．4．5569．R2=O．9983．byoptimizingtheconditionsofuse．AndMIPhasgoodeffectsofenrichment，whichCanmultiple48timesconcentration．Therefore，19-NT-MIPCanfullymeetthesolid-phaseextractionapplicationrequ

6、irements，SOCanbeprovidesfastandefficientmeansofsamplepre—treatmentforHPLC，ELISA，dipstickcolloidalgolddetectiontechnology．Keywords：19一NT：ELISA；colloidalgold；MIP；residuedetect主要缩略语19．去甲基睾酮(19-NT)牛血清蛋白(BSA)鸡卵清蛋白(OVA)酶联免疫吸附测定(ELISA)硝酸纤维膜(NC)分子印迹．(MIP)同相萃取(SPE)透射电镜(TEM)V目录目录

7、摘要⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯IAbstract⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯III目jj乏⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．I第一章绪论⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯11．1酶联免疫检测技术⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．11．2胶体金免疫层析检测技术⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯．31．3分子印迹检测技术⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯

8、⋯⋯⋯⋯⋯⋯⋯⋯⋯．51．3．1分子印迹的原理⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯51．3．2分子印迹聚合物的识别机理⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯61．3．3分子印迹技术分类⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯⋯