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1、入门指南简介和绝对定量绝对定量实验示例实验美国应用生物系统公司7300/7500实时定量PCR仪设计绝对定量实验PrimerExtendedonmRNA5′3′5′cDNAReverse执行反转录PrimerOligod(T)orrandomhexamerSynthesisof1stcDNAstrand3′5′cDNA运行绝对定量反应板分析绝对定量数据©Copyright2004,AppliedBiosystems.Allrightsreserved.ForResearchUseOnly.Notforuseindiagnosticproc
2、edures.AuthorizedThermalCyclerThisinstrument,SerialNo___________,isanAuthorizedThermalCycler.Itspurchasepriceincludestheup-frontfeecomponentofalicenseunderUnitedStatesPatentNos.4,683,195,4,683,202and4,965,188,ownedbyRocheMolecularSystems,Inc.,andundercorrespondingclaimsin
3、patentsoutsidetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd,coveringthePolymeraseChainReaction("PCR")processtopracticethePCRprocessforinternalresearchanddevelopmentusingthisinstrument.TherunningroyaltycomponentofthatlicensemaybepurchasedfromAppliedBiosystemsorobtainedbypur
4、chasingAuthorizedReagents.ThisinstrumentisalsoanAuthorizedThermalCyclerforusewithapplicationslicensesavailablefromAppliedBiosystems.ItsusewithAuthorizedReagentsalsoprovidesalimitedPCRlicenseinaccordancewiththelabelrightsaccompanyingsuchreagents.Purchaseofthisproductdoesno
5、titselfconveytothepurchaseracompletelicenseorrighttoperformthePCRprocess.FurtherinformationonpurchasinglicensestopracticethePCRprocessmaybeobtainedbycontactingtheDirectorofLicensingatAppliedBiosystems,850LincolnCentreDrive,FosterCity,California94404.DISCLAIMEROFLICENSE:No
6、rightsforanyapplication,includinganyinvitrodiagnosticapplication,areconveyedexpressly,byimplicationorbyestoppelunderanypatentorpatentapplicationsclaiminghomogeneousorreal-timedetectionmethods,includingpatentscoveringsuchmethodsusedinconjunctionwiththePCRprocessorotherampl
7、ificationprocesses.The5'nucleasedetectionassayandcertainotherhomogeneousorreal-timeamplificationanddetectionmethodsarecoveredbyUnitedStatesPatentNos.5,210,015,5,487,972,5,804,375and5,994,056,ownedbyRocheMolecularSystems,Inc.;bycorrespondingpatentsandpatentapplicationsouts
8、idetheUnitedStates,ownedbyF.Hoffmann-LaRocheLtd;andbyUnitedStatesPatentNos.5,538,848and6,030,787
