资源描述：
《Automatic Flow Procedure for the Determination.pdf》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、920FERNANDES&REIS:JOURNALOFAOACINTERNATIONALVOL.87,NO.4,2004FOODCOMPOSITIONANDADDITIVESAutomaticFlowProcedurefortheDeterminationofEthanolinWineExploitingMulticommutationandEnzymaticReactionwithDetectionbyChemiluminescenceELIZABETHN.FERNANDESUniversidadeFederald
2、eSoCarlos,DepartamentodeQuímica,POBox676,13565-905SoCarlos-SP-Brazil1BOAVENTURAF.REISUniversidadedeSoPaulo,CentrodeEnergiaNuclearnaAgricultura,POBox96,13400-970Piracicaba-SP-BrazilAnautomaticprocedureforthedeterminationofincludingseparationbydistillation,whi
3、chisaslowethanolinwinesusingaflowsystembasedonprocedure(2).Toimprovethesamplingrate,severalmulticommutationandenzymaticreactionisalternativeproceduresthatuseflowsystemhavebeendescribed.Alcoholoxidasewasimmobilizedonproposed(3–9).Someprocedureswereimplementedbyu
4、singaminopropylglassbeadsandpackedinanacrylicsample-handlingstrategies,suchasgas-diffusion(4)andcolumn.Theperoxideduetoenzymaticreactionpervaporation(5–7).withethanolreactedwithluminolandgeneratedRecently,enzymaticreactionshavebeenwidelyusedforthechemiluminesce
5、nceradiationthatwasanalysesbecauseoftheirsensitivityandselectivity.Proceduresmonitoredbyusingalaboratory-madedetectorforthedeterminationofethanolinwinesbasedonenzymaticbasedonphotodiodes.Thesystemmanifoldreactionsusingdifferentanalyticaltechniqueshavebeencompri
6、sedasetof3-waysolenoidvalvesproposed(1,4,8,10–13).Amongthem,thosebasedoncontrolledbyamicrocomputerfurnishedwithamperometryusingbiosensorstoexploitredoxreactionshaveelectronicinterfaces,whichranonsoftwarebeenpresentedasapromisinganalyticalalternative(10,12,13).w
7、ritteninQuickBASIC4.5toprovidefacilitiestoTheenzymaticproceduresforethanoldeterminationperformon-linesampledilution,reagentaddition,normallyhavebeenimplementedusingalcoholoxidaseanddataacquisition.Aftersystemparameters(AOD;10,11)andalcoholdehydrogenase(ADH;1,8,
8、12,13).optimization,ethanolsampleswereprocessedInthefirstcase,theethanolhasbeendeterminedbymeasuringwithoutpriorpretreatment.ThefollowingsuitableeitherconsumptionofO2orprodu
