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ID:33211318
大小:5.49 MB
页数:53页
时间:2019-02-22
《血管内皮生长因子复合细胞支架复合物对修复大鼠股骨缺损的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、江苏大学硕士学位论文Methods:RatMSCswereisolatedandpurifiedinvitro,andtheratMSCsof3rd-·generationwereseededontopreparednano--hydroxyapatite/collagen(nHAC).Theiradhesionsituationwasanalyzedbyscanningelectronmicroscope(SEM)after5,10days.Animalmodelsoffemoralbonedefectwereestablishedin30Sprague—Dawleyra
2、ts.Theyweredividedinto3groupsrandomly:IngroupA,asthecontrolgroup,defectwasfilledwithnHAC;ingroupBwithnHAC/MSCs;ingroupCwithVEGF/MSCs/nHAC.Animalsareexecutedinbatchpostoperative2,4,8,12weeks.Generalconditions,macroscopicevaluation,X—ray,thebonedensitymeasurementandhistomorphologyexamination
3、ofbonedefectrepairingsampleandstatisticalanalysiswereperformedinordertocomparetheeffectsofrepairingbonedefectsofthesegroups.Environmentscanningelectronmicroscope(ESEM)observationwasmade12weeksafteroperation.Theanimaldisposalwasconsistentwiththeethicalrequirements.Results:1.Ratbonemarrowmes
4、enchymalstemcells(MSCs)growadherent.Thespeedofgenerationisfast.ThegrowthcurveisfitforLogisticgrowthCUrVe.2.Ratbonemarrowmesenchymalstemcells(MSCs)andnano—hydroxyapatite/collagen(nHAC)arecultivatedinvitro,thescanningelectronmicroscope(SEM)isusedtoobserveat5m,10mday.Itcanbe江苏大学硕士学位论文seenthat
5、MSCscellpopulationadheredontonHACat10mday(38.52-士7.21)areobviouslymorethanat5‘hday(21.28i4.70),P6、rescenceiSfine.4.Macroscopicevaluation:ThebonedefectionofgroupCwascompletelyrepairedatpostoperative12weeks.TheprostheticconditionofbonedefectionwasobviouslybetterthangroupA,Bthosewerepartly5.Radiographicandradiographicscoring:IngroupC,therewerefewcallusformationsintheradialdefectsin2weeks;7、evidentbridgesgraftingwasobservedinthedefectsinthe8thweek;thedefectswerefilledwithnewlyformedbonecompletely12weeks.IngroupA,B,thereweremorecallusformationsin12weeks,butnobridgesgraftingwasobservedandtherewasosteosclerosisinthetwoends.Bonenonunioncanbeseenin2sa
6、rescenceiSfine.4.Macroscopicevaluation:ThebonedefectionofgroupCwascompletelyrepairedatpostoperative12weeks.TheprostheticconditionofbonedefectionwasobviouslybetterthangroupA,Bthosewerepartly5.Radiographicandradiographicscoring:IngroupC,therewerefewcallusformationsintheradialdefectsin2weeks;
7、evidentbridgesgraftingwasobservedinthedefectsinthe8thweek;thedefectswerefilledwithnewlyformedbonecompletely12weeks.IngroupA,B,thereweremorecallusformationsin12weeks,butnobridgesgraftingwasobservedandtherewasosteosclerosisinthetwoends.Bonenonunioncanbeseenin2sa
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