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大小:67.19 KB
页数:3页
时间:2019-02-15
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M方法A病名X药名C细胞SCI文章常用句型目的TodevelopthemethodsforisolationandcultivationofA.ToculturesthfromsurgicaliridectomyspecimensofeyeswithandwithoutA.TheeffectofvariousXsonthegrowth,melanogenesisanddendrificationofculturedCswerestudied.病因Asareinvolvedinthepathogenesisofavarietyofeyediseases,themostimportantofwhichisA,themostcommonintraoculartumor.AalsocontributetothepathogenesisofX1andX2.文献回顾Duringthepast2decades,muchworkhasbeendonetoisolateandcultivateC1andC2andtoinvestigatethefunctionofthesecellsinvitro.PGsplayimportantphysiopathologicalandtherapeuticrolesintheeye.SeveralPGshavebeenstudiedfortheireffectsonthereductionofIOPandtheirroleinthetreatmentofA.引起人们重视Duringthepastdecade,therehasbeenanincreasinginterestintherelationshipbetweenexposuretolightandthedevelopmentofcertaineyediseases,includingA1,A2,andA3.研究未明Little/(verylittle)isknownabout/(concerning)Therehavebeenonlyafewattemptsto---,andnosystematicattemptstoLittleworkhasbeenperformedon---Theroleofremainstobeelucidated/clarified.Comparedwithourknowledgeof---,littleisknownabout---.Evenlessisknownaboutits---.However,theeffectsof---havenotbeeninvestigatedthoroughly. M方法A病名X药名C细胞---hasneverbeensatisfactorilyexplained.Althoughmanystudiesof---havebeenreportedanditiswellestablishedthatEMdoproducemelanininvivo,littleisknownaboutmelanogenesisbyA.LittleworkhasbeenperformedonmelanogenesisbyAinvitro.InvestigationofmelanogenesisbyAinvitrohasbeenhamperedbyaninabilitytoobtainasufficientnumberofpureAforstudy.Theroleoftheirisinnormalfunctionandindiseaseremainstobeelucidated.仍有争议---remainscontroversial.WhetherAproducemelanininvivoduringadulthoodremainscontroversial.Theliteratureaboutthesthremainsscant.新进展SbhasdevelopedandreportherenewmethodsforAWehavedevelopedmethodsforARecentadvancesintechniquesof---suggested---Recently,ithasbeenfoundthatmelaninfromtheuveacouldstimulateA1andmaybeinvolvedinthepathogenesisofA2andtheA3.WehavedevelopedmethodsforisolationandcultureofA.保护作用Ocularpigmentation,particularly---,mayplayanimportantprotectiveroleinpreventionofthesediseases.研究目的ThepurposeofthepresentstudywastoinvestigatemelanogenesisbyAinvitro.WewantedtoexplorespecificallywhetherAproducemelanininvitro,whethermelanincontentandmelaninproductionbyAinvitrocorrelatewithirispigmentation,whether M方法A病名X药名C细胞culturedAexpress---activity,andwhether---activityofAcorrelateswith---,---and---.Wealsowantedtostudyfactorsinfluencingmelanincontentandproduction,aswellasthe---activityofAinvitro.比较分析RecentlyepidemiologicalandmolecularbiologicalstudiesindicatethatA1isdifferentfromA2andsimilartoA3,e.g.,theoccurrenceofA1isrelatedtothesunlightexposureandmutationofBRAFgene,whereastheC2arenot.ThedifferencebetweentheA1andA2maybedeterminedbythedifferentenvironmentalandcellularfactorsoftheC1andC2.TheseresultsindicatedthatXonlystimulatedcellgrowthatahighconcentration.AftersupplementationwithX,Agrowthwasstimulated,butmitoticactivitydeclinedrapidlyafterafewpassages.Theseeffectsweredose-dependentattherangefrom---to---M统计学ThedifferencesofXandcellcountsbetweenthecontrolandtestgroupswerestatisticallysignificant(cellnumber:0.05>P>0.01atconcentrationsof---Mincompletemedium,at---Min---medium,andP<0.01atconcentrationsof---Min---medium).ThedifferencesbetweentheXtreatedcultureandnegativecontrolsweresignificantat-6-5concentrationsof10-10MInthestudyon/(ofthe)cAMP-deletedmedium,thenumberofcellsincubatedwithXatallconcentrationsshowednosignificantdifferencecomparedwiththenegativecontrols.ThismethodprovidesavaluablesourceoflargenumbersofCs,whichcanbeusedtostudytheirbiologicalbehavior,tocomparewiththeA;andtocomparethemtotheirmalignantcounterpartsintheexplorationofthepathogenesisofA.
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